DEB-2菌株对贵州苹果早期落叶病病原的毒力效应

Toxicity of Endophyte DEB-2 to Pathogens of Guizhou Apple Early Defoliation Disease

为苹果早期落叶病的生物防治提供依据,采用组织分离法分离病原、形态与分子鉴定方法鉴定贵州苹果落叶病病原,用生物测定法研究DEB-2菌株对贵州苹果早期落叶病病原的毒力效应。结果表明:贵州苹果早期落叶病主要为苹果轮斑病和苹果斑点落叶病,其病原分别为轮斑病病原(Alternaria mali)和苹果斑点落叶病病原(Altemaria alternata);DEB-2菌株发酵液对A.mali和A.alternata抑制菌丝生长的EC50值分别为1.34%和0.12%;对抑制孢子萌发的EC50值分别为2.32%和5.32%。DEB-2菌株发酵液有效地抑制了贵州苹果早期落叶病原菌的菌丝生长和孢子萌发。

To provide a reference for biological control of apple early defoliation disease,pathogens were isolated using tissue separation method and identified by morphological and molecular methods.The virulence of endophyte DEB-2 against the pathogens was tested by bioassay method.The results showed that the pathogens of apple early defoliation disease in Guizhou were Alternaria alternata and Alternaria mali.The EC50 values of DEB-2 fermented liquid inhibiting mycelial growth of A.altenaata and A.mali were 1.34 ％ and 0.12 ％,respectively.The EC50 values of DEB-2 fermented liquid inhibiting spore germination ofA.alternata and A.maliwere 2.32 ％ and 5.32 ％,respectively.Therefore,endophyteic strain DEB-2 could effectively inhibit the mycelial growth and spore germ mination.