响应面优化超声辅助提取虎眼万年青多糖工艺

Optimization of Ultrasonic- assisted Extraction Process of Polysaccharides from Ornithogalum Caudatum Ait by Response Surface Methodology

采用响应面法优化虎眼万年青多糖的超声提取工艺。在单因素试验的基础上，采用Box—Benhnken试验设计优化超声时间（X1：20—40min）、提取温度（x2：50—70℃）、超声功率（Xs：900．1200W）和溶剂原料比（x4：30—50mL·g-1）对虎眼万年青多糖提取得率（Y）的影响。虎眼万年青多糖超声提取的最佳工艺条件为：超声时间30．05min、超声温度59．47℃、超声功率1050．15W、溶剂原料比40．11mL·g-1。在最佳的提取条件下，虎眼万年青粗多糖实验得率为50．2％，与模型预测值接近。应用响应面分析法优化得到的超声辅助提取参数准确、可靠。

Process of ultrasound - assisted extraction of polysaccharides from Ornithogalum Caudatum Ait was optimized by response sur- face methodology. Based on the single - factor experiments, the Box - Behnken design was employed to obtain best possible combination of extraction time （X1：20 - 40min）, extraction temperature （X2：50 -70℃）, ultrasound power （X3：900- 1200W） and solvent to the raw material ratio （X4：30 - 50mL·g-1） for maximum extraction yield of polysaccharides from Ornithogalum Caudatum Ait. The opti- mum extraction conditions were as follows： extraction time 30.05 min, extraction temperature 59.47℃, ultrasound power 1050. 15W and solvent to the raw material ratio 40. 11mL·g-1 Under these conditions, the experimental extraction yield was 50.2%, which matched the predicted value well. The parameters of ultrasound - assisted extraction optimized by response surface methodology were accurate and reliable.