电针治疗大鼠骨癌痛镇痛后效应的参数筛选

Screen the parameter of electroacupuncture's analgesic aftereffect on bone cancer pain

目的:观察不同频率频次电针多次治疗对大鼠骨癌痛的镇痛后效应以期筛选最佳参数。方法:实验完全随机分为假手术组、手术组、电针治疗组和假电针治疗组,电针治疗组又分为2Hz、100Hz和2/100Hz每日1次和隔日1次治疗6组。以胫骨内注入Walker256肿瘤细胞建立胫骨癌痛模型。电针治疗取双侧"足三里"和"昆仑"穴,1-2mA,30min/次。分别检测基础、造模后6d、治疗1d即刻、治疗2、4、6、8、10、12和14d后24h的痛阈。结果:与假手术大鼠比较,手术组大鼠在造模后各时间点的痛阈均低于假手术组(P<0.01)。与手术组大鼠比较,2Hz、100Hz和2/100Hz电针治疗后即刻,各治疗组大鼠痛阈明显升高(P<0.01),且各治疗组间痛阈无统计学差异;同一频次不同频率之间比较发现,2Hz、100Hz和2/100Hz电针每日治疗组各时间点均无明显的镇痛后效应;于治疗12d后24h,2Hz和2/100Hz电针隔日治疗组的痛阈明显高于手术组。同一频率不同频次电针治疗比较,2Hz、100Hz和2/100Hz电针在治疗一定天数(8d、6d、6d)之前,均是每日1次治疗效果较好,之后则为隔日1次治疗效果较好,但仅2Hz电针治疗有统计学意义。结论:单次电针治疗对骨癌痛有良好的镇痛作用,其镇痛作用与电针频率不相关;长期电针治疗,隔日电针治疗骨癌痛的镇痛后优于每日电针治疗,尤以2Hz、2/100Hz电针治疗12d后最佳。

Objective： We used different frequency and interval of electroacupuncture （EA） to treat bone cancer pain many times in order to select the best treatment parameters. Methods： All rats were randomly divided into sham surgery group, surgery group, EA group and sham EA group. EA group was further divided into 2Hz, 100Hz, 2/100Hz once a day （2Hz I , 100Hz I , 2/100Hz I ） and once every other day （2Hz Ⅱ, 100Hz Ⅱ, 2/100Hz Ⅱ）, six groups. Bone cancer pain model was established by implantation walker256 cell into tibial cavity. EA （dilatational wave, lmA for 15min, 2mA for 15min） was applied to bilateral ＇Zusanli＇ （ST 36） and ＇Kunlun＇ （BL 60） for 30min. The paw withdrawal thresholds （PWTs） were measured by using Dynamic Plantar Aesthesiometer 37450 before surgery （as Base） and at 6d after surgery, immediately after treatment 1 time, 2411 after treatment 2d, 4d, 6d, 8d, 10d, 12d and 14d. Results： Compared with sham group, PWTs of rats in CFA group were decreased markedly after surgery （P〈0.01）. Compared with surgery group, different parameters of EA （2Hz, 100Hz and 2/100Hz） treatment once could significantly increase the PWTs, and the PWTs did not have a difference among the three parameters. At same interval and different frequency of EA treatment, compared with surgery group, the PWTs of 2Hz I, 100Hz I and 2/100Hz I treatment did not change after 24h at 2d, 4d, 6d, 8d, 10d, 12d and 14d treatments, however, 2Hz Ⅱand 2/100HzⅡ could increase the PWTsafter 24h at 12d treatment. At different intervals and same frequency of EA treatment, early treatment （treatment of 2d and 4d） after 24h, the PWTs of 2Hz I, 100Hz I and 2/100Hz I respectively were higher that that of 2Hz Ⅱ, 100HzⅡ and 2/100Hz; however, 2Hz II, 100Hz Ⅱ and 2/100Hz II respectively were higher than that of 2Hz I, 100Hz I and 2/100Hz I at late treatment （treatment of 8d, 6d, 6d）. Conclusion： EA treatment once has a good analgesic effect on bone cancer pain, its analgesic effect is not related with EA frequency. EA treatment for a long time, the analgesic aftereffect of EA treatment by once every other day is better than EA treatment by once a day, especially after 12d of 2Hz or 2/100Hz treatment group.