平均临床浓度艾烟下的豚鼠全身过敏反应实验研究(英文)

Experiment study on systemic anaphylaxis reaction in guinea pigs under the average clinical concentration of moxa smoke

目的:观察平均临床浓度的艾烟是否引发豚鼠全身过敏反应,探讨艾烟的安全性。方法:选择健康无伤豚鼠8只,雌雄各4只。暴露于平均临床浓度的艾烟下进行致敏,艾烟浓度控制在3-4mg/m3之间,隔日1次,每次致敏20min,共3次。第3次致敏后第14天暴露于同浓度的艾烟40min进行激发。致敏期间密切观察每只动物的症状,激发后连续观察3h。并在第1、3次致敏和激发当天,称量记录体质量。结果:在整个致敏及激发致敏期间,豚鼠表现正常,未出现全身过敏反应症状,豚鼠体质量增加正常。结论:平均临床浓度的艾烟环境下不会引起豚鼠全身过敏反应。

Objective： To observe whether average clinical concentration of moxa smoke can cause systemic anaphylaxis reaction, and to evaluate the safety of moxa smoke. Methods： 8 healthy guinea pigs, 4 male and 4 female, were exposed to 3-4mg/m3 of moxa smoke. For sensitization, the animals were sensitized for 3 times, 20min each time every other day. For challenge, they were exposed to the same concentration of moxa smoke for 40min 14 days after the third sensitization. The symptoms were closely observed and recorded during sensitization period. Another 3 hours were required for observation after challenge. Every animal was weighed respectively on the days of the 1st sensitization, the 3rd sensitization and the challenge. Results： Animals were seen normally and there was no symptom of systemic anaphylaxis reaction during sensitization and challenge with normal body mass gain. Conclusion： The average clinical concentration of moxa smoke can not result in systemic anaphylaxis reaction in guinea pigs. Key words： Moxa smoke; Guinea pigs; Systemic anaphylaxis reaction; Sensitization; Challenge