系统性红斑狼疮患者外周血淋巴细胞亚群分析

Analysis of the lymphocyte cell subsets in peripheral blood of patients with systemic lupus erythematosus

目的探讨T淋巴细胞亚群、B淋巴细胞和NK细胞在系统性红斑狼疮(SLE)中的作用。方法利用流式细胞仪对29例活动期、24例非活动期SLE患者及50例健康对照者的外周血中的CD3+、CD4+、CD8+、CD3-CD16+CD56+(NK)及CD19+(B)淋巴细胞进行检测。结果活动期组与非活动期组及健康对照组比较:CD4+T淋巴细胞、CD4+/CD8+比值及NK细胞明显降低(P<0.05),B淋巴细胞明显增高(P<0.05),CD3+T和CD8+T淋巴细胞无统计学意义(P>0.05)。非活动期组与健康对照组比较,T淋巴细胞各亚群、NK细胞及B淋巴细胞均无统计学意义(P>0.05)。结论检测外周血淋巴细胞亚群,对判断病情及指导临床治疗具有重要意义。

Objective To investigate the role of T lymphocyte cell subsets, B cells and NK cells in systemic lupus erythe- matosus （SLE）. Methods A total of 29 SLE patients in active phase, 24 SLE patients in quiescent phase and 50 healthy controls were enrolled. The CD3＋, CD4＋, CD8＋, CD3-CD16＋CD56＋ （NK） and CD19＋（B） cells in peripheral blood of SLE patients and healthy controls were detected by flow cytometry. Results Compared with the SLE patients in quiescent phase and healthy controls, there was significant difference in CD4＋, CD4＋/CD8＋, B and NK cells in active phase SLE patients（P〈0.05）, and no significant difference in CD8＋ cells in active phase SLE patients （P〉0.05）.There was no significant difference in CD3＋,CD4＋,CD8＋, CD3-CD16＋CD56＋（NK） and CD19＋（B） cells between quiescent phase SLE patients and healthy controls. Conclusion Lymphocyte cell subsets may play an important role in the pathogenesis, progression and therapy of SLE.