摘要
[ Objective] The research aimed to develop a new molecular imprinting solid-phase extraction column for the separation and enrichment of melamine in feed. [ Method] The molecular imprinting polymer of melamine was prepared by mass polymerization method. This polymer was used as filler to prepare molecular imprinting polymer solid-phase extraction(MIP-SPE) column of melamine. The solid-phase extraction conditions were optimized and melamine content in concentrated solution was determined by using HPLC. [ Result] Melamine eould be separated selectively from feed and enriehed by self-made MIP-SPE column. It could wipe off complex matrix from feed and the addition recovery rate was 95%. The contrast experiment between MIP-SPE column and NMIP-SPE ( non-imprinted polymer- solid phase extraction) column showed that the extraction efficiency of the former was obviously higher than the latter. [ Conclusion] The home-made MIP- SPE column could be used to separate and enrich melamine in feed efficiently, with a broad application prospect.
[ Objective] The research aimed to develop a new molecular imprinting solid-phase extraction column for the separation and enrichment of melamine in feed. [ Method] The molecular imprinting polymer of melamine was prepared by mass polymerization method. This polymer was used as filler to prepare molecular imprinting polymer solid-phase extraction(MIP-SPE) column of melamine. The solid-phase extraction conditions were optimized and melamine content in concentrated solution was determined by using HPLC. [ Result] Melamine eould be separated selectively from feed and enriehed by self-made MIP-SPE column. It could wipe off complex matrix from feed and the addition recovery rate was 95%. The contrast experiment between MIP-SPE column and NMIP-SPE ( non-imprinted polymer- solid phase extraction) column showed that the extraction efficiency of the former was obviously higher than the latter. [ Conclusion] The home-made MIP- SPE column could be used to separate and enrich melamine in feed efficiently, with a broad application prospect.
基金
Supported by Xiamen Science and Technology Plan Project(3502Z20123046)
A-class Plan Project of Education Department of Fujian Province(JA12465)