缺氧诱导因子1α和碳酸酐酶Ⅸ在宫颈上皮癌变过程中的表达及意义

Expressions and clinical significance of hypoxia inducible factor 1α and carbonic anhydrase Ⅸ in cancerization of cervical intraepithelial neoplasia

目的探讨缺氧诱导因子1α(HIF-1α)和碳酸酐酶Ⅸ(CAⅨ)表达在宫颈上皮癌变过程中的意义。方法经术后病理检查确诊宫颈病变患者89例:宫颈上皮内瘤样病变(CIN)Ⅰ-Ⅱ级28例,CINⅢ级20例,宫颈鳞癌41例;对照组为正常宫颈组织22例。应用SP免疫组化法检测HIF-1α和CAⅨ表达,分析HIF-1α和CAⅨ与宫颈癌临床病理特征间的关系。结果宫颈鳞癌、CINⅢ和CINⅠ-Ⅱ组织中的HIF-1α阳性表达率分别为75.6%、50.0%和32.1%(P<0.05),CAⅨ的阳性表达率分别为73.2%、65.0%和39.3%(P<0.05)。宫颈鳞癌组织中HIF-1α和CAⅨ的阳性表达率随临床分期的升高而增加(P<0.05);HIF-1α和CAⅨ蛋白在宫颈鳞癌中的阳性表达率呈正相关(r=0.682,P<0.05)。结论 HIF-1α和CAⅨ在CIN和宫颈癌组织中呈高表达,提示检测HIF-1α和CAⅨ对宫颈癌早期诊断有临床意义。

Objective To study the expressions and clinical significance of hypoxia inducible factor 1α（HIF-1α） and carbonic anhydrase IX （CA IX） in the cancerization of cervical intraepithelial neoplasia（CIN）. Methods Imrnunohistochemical SP method was used to detect the expressions of HIF-1α and CAD[ in normal cervix tissues（22 cases）, CIN I- II （28 cases）, CIN III （20 cases） and cervical squamous cell carcinoma （41 cases）. The diagnosis was confirmed pathologically after operation. The correlations between the expressions of HIF-1α and CA Ⅸ and clinicopathologicaal characteristics were analyzed. Results The expression of HIF-1α was 75.6% in cervical carcinoma, 50.0% in CIN III and 32.1% in CIN I-II （P〈0. 05）. The expression of CA IX was 73.2% in cervical carcinoma, 65.0% in CIN III and 39.3% in CIN I -II （P〈0. 05）. The positive expresstions of HIF-1α and CAIX in cervical squamous cell carcinoma increased as its clinical stage elevated. The expression of HIF-la in cervical squamous cell carcinoma was positively correlated with that of CA IX （r=0. 682,P〈0. 05 ）. Conclusion The HIF-1α and CAIX are highly expressed in CIN and cervical carcinoma, indicating that detection of HIF-1α and CAIX plays an important role in early diagnosis of cervical carcinoma.