解脲脲原体血清1型与8型对小鼠生殖道的致病性研究

Pathogenicity of Ureaplasma urealyticum serotype 1 and 8 in the genital tract of female BALB/c mice

目的探讨相同条件下，解脲脲原体血清1型（Upl）和8型（Uu8）在BALB／c小鼠生殖道感染模型中的致病性。方法48只BALB／c小鼠随机分为空白对照组、雌激素组、Upl感染组、Uu8感染组，每组12只。分别接种无菌液体培养基、Upl、Uu8标准菌液。接种后第3、7、14、21天，每组随机取3只小鼠，采集阴道灌洗液后处死，取阴道、子宫标本固定后，HE染色镜检。阴道灌洗液用于培养和肿瘤坏死因子（TNF）一仅检测。结果接种后空白组和雌激素组均无解脲脲原体生长，TNF-d为（4．17±0．85）pg／ml。两个感染组各时间点小鼠阴道冲洗液经培养均为阳性，随着时间延长，测定颜色改变单位逐渐下降。TNF-d在感染后第14天达峰值，分别为（14．93±1．11）pg／ml和（27．04±24．26）pg／ml。组织病理结果显示，Upl和Uu8感染后均可导致急慢性炎症反应，以子宫病变为主，Upl可导致官腔粘连。结论BALB／c小鼠下生殖道感染Upl和Uu8后，主要导致宫颈炎，两组致病性未见明显差异。Upl可能与宫腔粘连有关。

Objective To compare the pathogenicity between Ureaplasma urealyticum serotype 1 （Upl） and 8 （Uu8） in the genital tract of BALB/c mice. Methods A total of 48 BALB/e mice were randomly and equally divided into four groups： blank control group receiving no treatment, estradiol group pretreated with intramuscular injection of estradiol followed by intravaginal inoculation with sterial liquid culture media, Upl and Uu8 groups pretreated with intramuscular injection of estradiol followed by intravaginal inoculation with suspensions of Upl and Uu8 respectively. Three mice were randomly selected from each group to be sacrificed after the collection of vaginal lavage fluid on day 3, 7, 14 and 21 after the inoculation. Vaginal and uterine tissue specimens were obtained from these sacrificed mice and underwent hematoxylin and eosin （HE） staining. Vaginal lavage fluid samples were subjected to culture of Uu and measurement of tumor necrosis factor-or （TNF-ot）. Results No evidences were observed for Uu growth in either the blank control group or estradiol group at any of the time points after the inoculation, with the average level of TNF-ct in vaginal lavage fluid being （4.17 ± 0.85） pg/ml at these time points in both groups. Uu grew in all the vaginal lavage fluid samples from the Up1 and Uu8 groups at the four time points, with the color change unit （CCU） value decreasing with time. The level of TNF-ot in vaginal lavage fluid peaked on day 14 after the inoculation in the Upl （（ 14.93 ± 1.11 ） pg/ml） and Uu8 （（27.04 ± 24.26） pg/ml） groups. Both Upl and Uu8 infection caused acute and chronic inflammatory responses in the mice, which were mainly located in the uterus, and Upl might cause intrauterine adhesion. Conclusions At the same inoculation concentration, no significant difference is found in the pathogenicity between Upl and Uu8, both of which appear to mainly cause cervicitis. Upl might be partially responsible for intrauterine adhesion in mice.