摘要
目的探讨IL-10在乳腺癌患者肿瘤组织中的表达与临床病理特征、预后的关系。方法回顾性收集2000—01/2002—12期间在解放军总医院接受手术的130例I-Ⅲ期乳腺癌患者的临床资料及石蜡切片,用免疫组织化学法检测乳腺癌原发灶IL-10蛋白的表达,分析IL-10表达与乳腺癌临床病理特征及预后的关系。结果IL-10在肿瘤细胞及肿瘤间质细胞中均有表达。肿瘤细胞IL-10高表达与较小的肿瘤直径、较高的肿瘤分化程度、ER阳性和脉管癌栓阴性相关(P〈0.05或P〈0.01);在COX多因素预后分析中,肿瘤细胞质IL-10染色强度是无病生存期的独立预后因素(HR=0.443,P=0.022),IL-10+间质细胞密度是总生存期的独立预后因素(HR=0.411,P=0.051)。结论IL-10在乳腺肿瘤细胞和间质的表达强度可作为乳腺癌预后的预测指标。较低的IL-10表达水平与乳腺癌较差的预后有关。
Objective To evaluate the prognostic value of IL-10 expression in breast cancer patients and determine the correlation between IL-10 expression and clinicopathological features of breast cancer. Methods Paraffin sections were retrospectively collected from 130 patients of stage I-III breast cancer who received surgery between January 2000 and December 2002 in the Chinese PLA General Hospital. The protein expression of IL-10 in the primary lesions was detected by immunohistochemistry to analyze the correlations of IL-]0 expression with clinicopathological variables and prognosis. Results IL-10 was found expressed in the cytoplasm of both tumor cells and stromal cells. Higher IL-10 expression in tumor cell cytoplasm was associated with lower T grade ( P = 0. 012), higher SBR grade ( P = 0. 004), positive ER ( P = 0. 007 ), negative vascular invasion ( P = 0.034). The multivariant COX regression analysis showed that higher IL-10 expression in tumor cell cytoplasm was associated with better disease-free survival ( HR = 0.443, P = 0. 022) and that higher density of IL-10+ in stromal cell cytoplasm was associated with overall survival (HR =0.411, P = 0.051 ). Conclusion IL-10 expression level in breast tumor cells and stromal cells can be used as a predictor of breast cancer prognosis. Low expression of IL-10 is associated with poor survival outcome.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2014年第5期517-520,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
吴阶平医学基金(320.6752.1230)