乳腺癌患者肿瘤组织IL-10的表达及其与预后的关系

Prognostic value of IL-10 expression in tumor tissues of breast cancer patients

目的探讨IL-10在乳腺癌患者肿瘤组织中的表达与临床病理特征、预后的关系。方法回顾性收集2000—01／2002—12期间在解放军总医院接受手术的130例I-Ⅲ期乳腺癌患者的临床资料及石蜡切片，用免疫组织化学法检测乳腺癌原发灶IL-10蛋白的表达，分析IL-10表达与乳腺癌临床病理特征及预后的关系。结果IL-10在肿瘤细胞及肿瘤间质细胞中均有表达。肿瘤细胞IL-10高表达与较小的肿瘤直径、较高的肿瘤分化程度、ER阳性和脉管癌栓阴性相关（P〈0．05或P〈0．01）；在COX多因素预后分析中，肿瘤细胞质IL-10染色强度是无病生存期的独立预后因素（HR=0．443，P=0．022），IL-10＋间质细胞密度是总生存期的独立预后因素（HR=0．411，P=0．051）。结论IL-10在乳腺肿瘤细胞和间质的表达强度可作为乳腺癌预后的预测指标。较低的IL-10表达水平与乳腺癌较差的预后有关。

Objective To evaluate the prognostic value of IL-10 expression in breast cancer patients and determine the correlation between IL-10 expression and clinicopathological features of breast cancer. Methods Paraffin sections were retrospectively collected from 130 patients of stage I-III breast cancer who received surgery between January 2000 and December 2002 in the Chinese PLA General Hospital. The protein expression of IL-10 in the primary lesions was detected by immunohistochemistry to analyze the correlations of IL-]0 expression with clinicopathological variables and prognosis. Results IL-10 was found expressed in the cytoplasm of both tumor cells and stromal cells. Higher IL-10 expression in tumor cell cytoplasm was associated with lower T grade （ P = 0. 012）, higher SBR grade （ P = 0. 004）, positive ER （ P = 0. 007 ）, negative vascular invasion （ P = 0.034）. The multivariant COX regression analysis showed that higher IL-10 expression in tumor cell cytoplasm was associated with better disease-free survival （ HR = 0.443, P = 0. 022） and that higher density of IL-10＋ in stromal cell cytoplasm was associated with overall survival （HR =0.411, P = 0.051 ）. Conclusion IL-10 expression level in breast tumor cells and stromal cells can be used as a predictor of breast cancer prognosis. Low expression of IL-10 is associated with poor survival outcome.