近红外荧光分子成像在裸鼠卵巢癌移植瘤模型中的应用

Application of near-infrared fluorescence molecular imaging in nude mice xenograft models of ovarian cancer

目的观察近红外荧光吲哚七甲川菁染料IR-783在裸鼠卵巢癌移植瘤模型中对肿瘤的特异靶向作用。方法采用卵巢癌细胞株SKOV-3创建卵巢癌裸鼠模型,将IR-783注入15只裸鼠体内,之后采用MaestroTM2.10活体成像系统分别在30min、1h、6h、24h、48h观察染料的肿瘤特异靶向作用及其在荷瘤裸鼠体内的代谢分布。结果图像分析软件测得肿瘤平均体积(16.34±3.35)cm3,游标卡尺测得为(15.56±1.85)cm3(P=0.79);注射后30minIR-783主要分布于肝脏、心脏、肾脏和肺,6h肿瘤荧光强度达到峰值,注射后48h肿瘤部位仍存在少量荧光信号。结论近红外荧光染料IR-783可无创、准确、直观地反映裸鼠卵巢癌移植瘤的生长。

Objective To observe the uptake and retention of heptamethine dyes （IR-783） in nude mice xenograft models of ovarian cancer. Methods The ability of IR-783 to accumulate in human ovarian cancer cell SKOV-3 was investigated. Fifteen nude mice （n=15） were inoculated with cells, and imaging （MaestroTM 2.10） in vivo and ex vivo were performed periodically after 30 min, 1 h, 6 h, 24 h and 48 h, respectively. Results The average volume detected by imaging analysis software was （16.34±3.35）cm3 , by calipers was （15.56± 1.85） cm3 （P=0.79）. IR-783 accumulated mainly in liver, kidney, heart and lung in the first 30 min. The mean fluorescent intensity gradually increased and reached the maximum at 6 h, while there was still a small amount of tumor fluorescence signals after 48 h. Conclusion IR-783 could be used in e- valuation on nude mice xenograft models of ovarian cancer.