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肽酰基精氨酸脱亚胺酶4在ANCA相关性小血管炎中的作用探讨 被引量：4

The significance of serum peptidylarginine deiminase type 4 in ANCA- associated vasculitis
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摘要 目的通过检测肽酰基精氨酸脱亚胺酶4（PAD4）在ANCA相关性小血管炎（AAV）患者血清中的水平，探讨其在AAV发病中的作用及意义。方法收集13例AAV患者发作期与缓解期血清，11例原发性慢性肾脏病患者的血清，12例健康对照的血清。用酶联免疫吸附法（ELISA）检测PAD4的水平，分析其与AAV患者BVAS评分的关系。结果（1）在AAV组，PAD4在发作期与缓解期的水平明显高于健康对照组；而在慢性肾脏病患者组中，PAD4水平与健康对照相比无显著差异。（2）AAV组有肾损害的患者PAD4水平，无论发作期还是缓解期，均高于慢性肾脏病组患者的PAD4水平。（3）AAV肾损害组患者发作期的PAD4水平与BVAS评分呈显著正相关（r=0．71，P=0．02）。结论PAD4参与了AAV的发生和发展。 Objective To explore the significance of peptidylarginine deiminase type 4 （PAD4） in the pathogenesis of ANCA-associated vasculitis （AAV） by detecting its level in patients with AAV. Methods Sera from 13 patients with AAV, 11 patients with primary chronic kidney disease and 12 healthy controls were collected. Serum PAD4 was detected using commercial ELISA kits. The association between serum PAD4 and BVAS of AAV was further investigated. Results （1） The serum level of PAD4 in patients with AAV in active and remission stages were all higher than that in the healthy controls. The serum level of PAD4 in patients with CKD was not found elevated compared with the normal controls. （2） The serum levels of PAD4 in AAV with renal damage were all significantly higher than that in CKD group no matter in active or remission stage. （3） The serum level of PAD4 in AAV with renal damage in active stage was positively correlated with BVAS （r = 0.71, P= 0.02）. Conclusion PAD4 is involved in the pathogenesis of AAV.
作者 王增玲 李海娜 崔莉 邢广群
机构地区 青岛大学附属医院黄岛院区肾病科
出处 《中华肾脏病杂志》 CAS CSCD 北大核心 2014年第5期327-331,共5页 Chinese Journal of Nephrology
关键词 血管炎 抗体 抗中性白细胞胞质 中性粒细胞细胞外诱捕网 肽酰基精氨酸脱亚胺酶4 Vasculitis Antibodies, antineutrophil cytoplasmic Antineutrophil extracelluartraps Peptidylarginine deiminase type 4
分类号 R692 [医药卫生—泌尿科学] R593.2 [医药卫生—内科学]
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参考文献24

  • 1Kessenbrock K, Krumbholz M, Schonermarck U, et al. Netting neutrophils in autoimmune small-vessel vasculitis[J]. Nat Med, 2009, 15: 623-625.
  • 2邢广群,李敏,魏志敏.中性粒细胞细胞外网络在ANCA相关性小血管炎患者肾组织中的表达及意义[J].中国中西医结合肾病杂志,2012,13(11):960-962. 被引量：5
  • 3Li P, Li M, Lindberg MR, et al. PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps[J]. J Exp Med, 2010, 207: 1853-1862.
  • 4Nakashlma KH, Hagiwara T, Yamada M. Nuclear localization of peptidylarginine deiminase Vand histone deimination in granulocytes[J]. J Biol Chem, 2002, 277: 49562-49568.
  • 5Brinkmann V, Reichard U, Goosmann C, et al. Neutrophil extracellular traps kill bacteria[J]. Science, 2004, 303: 1532- 1535.
  • 6Papayannopoulos V, Zychlinsky A. NETs: a new strategy for using old weapons[J]. Trends Immunol, 2009, 30: 513-521.
  • 7Hunder GG, Arend WP, Bloch DA, et al. The American College of Rheumatology 1990 criteria for the classification of vasculitis. Introduction[J]. Arthritis Rheum, 1990, 33: 1065- 1067.
  • 8Jeanette JC, Falk R J, Andrassy K, et al. Nomenclature of systemic vasculitides, proposal of an International Consensus Conference[J]. Arthritis Rheum, 1994, 37: 187-192.
  • 9Luqmani RA, Bacon PA, Moots RJ, et al. Birmingham vasculitis activity score (BVAS) in systemic necrotizing vasculitis[J]. QJM, 1994, 87: 671-678.
  • 10Nakashima KT, Hagiwara A, Ishigami S, et al. Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and lalpha, 25-dihydroxyvitamin D(3) [J]. J Biol Chem, 1999, 274:27786-27792 .

二级参考文献14

  • 1Brinkmann V, Reichard U, Goosmann C, et al. Neutrophil extra- cellular traps kill bacteria. Science, 2004,303 ( 5663 ) : 1532 - 1535.
  • 2Brinkmann V, Zychlinsky A. Beneficial suicide:why neutrophils die to make NETs. Nat Rev Microbiol, 2007,5 ( $ ) :577 - 582.
  • 3Neeli I, Khan SN, Radic M, et al. Histone Deimination As a Re- sponse To Inflammatory Stimuli in Neutrophils. J Immuno,2008, 180 (3) : 1895 - 1902.
  • 4Kessenbrock K, Krumbholz M, Schnermarck U, et al. Netting neutrophils in autoimmune small - vessel vasculitis. Nat Med, 2009,15 (6) :623 - 625.
  • 5Clark SR, Ma AC, Tavener SA, et al. Platelet TLR4 activates neutrophic extracellular traps to ensnare bacteria in septic blood. Nat Med,2007,13 (4) :463 - 469.
  • 6Villanueva E, Yalavarthi S, Berthier CC, et al. Netting neutro- phils induce endothelial damage, infiltrate tissues, and expose immunostimulatory molecules in systemic lupus erythematosus. J Immunol, 2011,187 ( 1 ) :538 - 552.
  • 7Brinkmann V,Reichard U,Goosmann C. Neutrophil extracellular traps kill bacteria[J].Science,2004.1532-1535.doi:10.1126/science.1092385.
  • 8Brinkmann V,Zychlinsky A. Beneficial suicide:why neutrophils die to make NETs[J].Nat Bey Microbiol,2007,(8):577-582.doi:10.1038/nrmicro1710.
  • 9Neeli I,Khan SN,Radic M. Histone deimination as a response to inflammatory stimuli in neutrophils[J].Journal of Immunology,2008.1895-1902.
  • 10Hakkim A,Barbara G,Amann K. Impairment of neutrophil extracellular trap degradation is associated with lupus nephritis[J].Proceedings of the National Academy of Sciences(USA),2010.9813-9818.doi:10.1073/pnas.0909927107.

共引文献9

同被引文献35

  • 1Berden AE, Ferrario F, Hagen EC, et al. Histopathologic classification of ANCA-associated glomerulonephritis [J]. J Am Soc Nephrol, 2010, 21(10): 1628-1636.
  • 2Paassen P, Tervaert JW, Heeringa P. Mechanisms of vasculi- tis: how pauci-immune is ANCA-associated renal vasculitis? [J]. Nephron Exp Nephrol, 2007, 105(1): 10-16.
  • 3Sinico RA, Radice A. Antineutrophil cytoplasmic antibodies (ANCA) testing: detection methods and clinical application [J]. Clin Exp Rheumatol, 2014, 32(3 Suppl 82): S112-S117.
  • 4Kain R, Exner M, Brandes R, et al. Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis [ J ]. Nat Med, 2008, 14(10): 1088-1096.
  • 5Sedimbi SK, Hagglof T, Karlsson MC. IL-18 in inflammatory and autoimmune disease[J]. Cell Mol Life Sci,2013, 70(24) : 4795-4808.
  • 6Jennette JC, Falk RJ, Bacon PA, et al. 2012 revised interna- tional Chapel Hill Consensus Conference Nomenclature of Vas- culitides[J]. Arthritis Rheum, 2013, 65(1): 1-11.
  • 7Volin MV, Koch AE. Interleukin-18: a mediator of inflamma- tion and angiogenesis in rheumatoid arthritis[J]. J Interferon Cytokine Res, 2011, 31(10): 745-751.
  • 8Lourenco EV, La CavaA. Cytokines in systemic lupus erythe- matosus[J]. Curt Mol Med, 2009, 9(3) : 242-254.
  • 9Rasmy H, Mikhael N, Ismail S. Interleukin-18 expression and the response to treatment in patients with psoriasis[J]. Arch Med Sci, 2011, 7(4): 713-719.
  • 10Shui HA, Ka SM, Wu WM, et al. LPS-evoked IL-18 expres- sion in mesangial cells plays a role in accelerating lupus nephri- tis[J]. Rheumatology(Oxford), 2007, 46(8): 1277-1284.

二级引证文献5

中华肾脏病杂志
2014年 第5期

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