Cyclin D1在EGCG抗鼻咽癌中表达的变化及意义

Signifinace of Cyclin D1 expression in CNE2 cells processed by EGCG

目的:探讨Cyclin D1基因在表没食子儿茶素没食子酸酯(EGCG)抗鼻咽癌中表达的变化及意义,揭示EGCG的抗鼻咽癌作用机制。方法:体外培养低分化鼻咽癌细胞株CNE-2并以不同浓度EGCG处理,倒置显微镜下观察不同浓度EGCG作用48h后CNE-2细胞的形态变化,采用MTT比色法检测细胞增殖抑制率,流式细胞仪检测细胞周期,半定量逆转录PCR检测细胞中Cyclin D1mRNA的表达变化。结果:EGCG处理后,CNE-2细胞的数量及密度逐渐降低,分裂象减少,贴壁差,部分细胞变圆且体积变小,漂浮及凋亡细胞不断增多;细胞增殖明显受到抑制,CNE-2细胞被阻滞在G0/G1期,呈时间和剂量依赖性(P<0.05);Cyclin D1mRNA表达明显下调,且EGCG作用浓度与时间依赖性(P<0.05)。结论:EGCG抑制CNE-2细胞的增殖,可能与其呈浓度与时间依赖性下调Cyclin D1mRNA的表达相关。

Objective： To study the expression of Cyclin D1 in nasopharyngeal carcinoma cells processed by epigallocatechin gallate（EGCG） and it＇s significance, and revealed the anti-tumor mechanism of EGCG against na- sopharyngeal carcinoma. Method：CNE-2 cells were treated by EGCG at different concentrations, the morphological changes of CNE-2 cells were observed by inverted microscope; the inhibition ratio of cell proliferation was detected by MTT colorimetric method, flow cytometry was used to analyze the changes of cell cycle. The expression of Cyclin D1 mRNA was detected by RT-PCR. Result： After treated by EGCG, the CNE2 cells decreased in amount and density,some of which became roll and small; Floating and dead cells can be seen in the inverted microscopy; cell proliferation was significantly inhibited in a time and dose dependent （P〈0.05）. CNE-2 cells were arrested at G1/G0 phase. The expression of Cyclin D1 mRNA was down-regulated by EGCG with concentration and action time dependent （P〈0.05）. Conclusion： EGCG resisted nasopharyngeal carcinoma by inhibiting the cell proliferation, The down regulation of Cyclin D1 mRNA expression in a time and dose dependent may be the possible mechanisms.