抗菌药物对氟康唑动态抗真菌作用及透过生物膜的影响

The Impact of Antibacterial Agents on Dynamic Antifungal Effects of Fluconazole and Biofilm Penetration

目的:测定试验用抗菌药物与氟康唑联合的动态抗真菌作用,并评价试验用抗菌药物对氟康唑透过生物膜的影响。方法:采用活菌计数法描绘联合用药对白色念珠菌的动态杀菌曲线,以"三明治"透膜实验法评价试验用抗菌药物对氟康唑透过生物膜的影响。结果:试验抗菌药物对氟康唑有不同程度的增效作用,其中米诺环素和利福平对氟康唑透过耐药白色念珠菌生物膜的作用及其对氟康唑动态抗真菌的增效作用最强,氧氟沙星、阿奇霉素等次之。结论:试验用抗菌药物能促进氟康唑透过白色念珠菌生物膜,增强氟康唑的动态抗真菌作用。

Objective：To determine the combined dynamic antifungal effects of fluconazole and the tested antibacterial agents and evaluate the impact of the tested antibacterial agents on fluconazole penetration through biofilm. Methods：The dynamic killing curve was obtained based on the combined drugs against Candida albicans using viable bacterial counting method. The biofilm penetration test was evaluated by “sandwich” experimental method. Results：Synergistic effects on fluconazole were showed by using the tested antibacterial agents. The penetrating ability of fluconazole through biofilm by the drug resistant Candida albicans varied by different tested antibacterial drugs ,of which minocycline and rifampicin shown strongest impact on it but also on the synergistic effects of dynamic antifungal action. Levofloxacin ,azithromycin and others had a lower impact. Conclusion：The tested antibacterial agents can promote the fluconazole penetration of Candida albican ’ s biofilm and increase fluconazole’s dynamic antifungal effect.