灵芝三萜酸组分高效液相色谱指纹图谱初探

Preliminary Study on HPLC Fingerprint of Triterpene acids Components from Ganoderma Lucidum

目的:建立灵芝三萜酸组分(GLA)的指纹图谱,为其质量控制提供依据。方法:使用植物组织破碎法提取GLA,利用高效液相色谱法建立GLA的指纹图谱。结果:GLA高效液相色谱指纹图谱中有8个峰为其主要特征,其α值分别为0.68,0.73,0.90,0.95,1.00,1.12,1.36,1.46。结论:所建立的RP-HPLC指纹图谱分析方法可为GLA的质量控制提供理论依据。

Objective： To set up the fingerprint of triterpene acids components of ganoderma lucidum （GLA）and to provide a basis for its quality control. Methods： To extract the triterpene acids components of ganoderma lucidum by plant tissue crushing method and to establish the fingerprint of GLA by HPLC. Results： There were 8 characteristic peaks in HPLC fingerprint of GLA,their alpha values were 0.68,0.73,0.90,0.95,1.00,1.12,1.36 and 1.46 respectively. Conclusion：The established RP-HPLC fingerprint can provide a theoretical basis for the quality control of GLA.