枇杷花水提物防治小鼠急性酒精性肝损伤研究

Protective Effect of Aqueous Extract of Loquat Flower on Acute Alcohol-induced Liver Injury in Mice

目的初步观察枇杷花水提物(AELF)对急性酒精染毒小鼠的防治效果。方法取雄性昆明种小鼠,随机分为空白组、模型组、联苯双酯阳性药组、不同剂量AELF(400、800、1600 mg·kg-1)组,每组10只。按0.10 ml/10 g剂量灌胃给药10 d,末次给药2 h后一次性灌胃给予50%(V/V)无水乙醇溶液0.12 ml/10 g,禁食不禁水16 h。测定外周血象,血清中ALT、AST、ADH活性及肝匀浆中MDA、TG含量。结果与模型组相比,AELF各剂量组能明显降低WBC、AST、血清及肝脏TG水平,均以中剂量效果最好(P<0.01,P<0.01,P<0.01,P<0.05);高剂量组能够明显降低血清ALT及肝脏MDA水平(P<0.01,P<0.05)。结论 AELF可以通过降低染毒小鼠转氨酶和TG水平,提高机体抗氧化能力,对小鼠急性酒精性肝损伤起到一定的预防性保护作用。

Objective To investigate the effect of the aqueous extract of loquat flower （AELF） on acute alcoholic liver injury in mice. Methods The mice were randomly divided into 6 groups：control group, model group, positive drug group （Bifendate） ,and AELF groups at different doses （400,800,1600 mg·kg^-1）. Each group has 10 mice. Drugs were given once a day for 10 consecutive days,0.10 ml/10 g a time. 50% （ V/V ） dehydrated alco-hol of 0.12 ml/10 g was fed orally 2 h after the last administration except in control group. Then the animals were fasted for 16 h with water drinking allowed. Results Compared with model group, the AELF group at the dose of 800 mg ·kg^-1 underwent a marked decrease in the level of aspartate amino transferase （AST）, triglyceride （TG） of the serum and liver（ P 〈0.01, P 〈0.01, P 〈0.05） and the amount of WBC returned to normal（ P 〈0.01）, while in the 1600 mg·kg^-1 group the level of alanine amino transferase （ALT） in serum and the concentration of malondialdehyde （MDA） in liver homogenates was reduced significantly （ P 〈 0.01, P 〈 0.05 ）. Conclusion AELF can lower the level of ALT, AST, TG and improve the anti-oxidation ability of organisms. It has proactively protective effect on acute alcohol-induced liver injury in mice.