摘要
目的:解决脂蛋白(a)抗体批间差大,以及试剂盒检测结果不一致的问题。方法:制备脂蛋白(a)单克隆抗体,筛选与Kringle IV-2无反应,且能自身配对的杂交瘤细胞株,并对抗体识别位点与检测特异性进行分析。结果:获得了一株识别Kringle IV-5的单抗,标记为LPa-3,该单抗能够实现自身配对,用LPa-3单抗作为包被抗体与标记抗体初步建立的夹心ELISA方法检测15份血清样品,检测结果与商品试剂盒具有较好的相关性:y=0.190 4 x-0.032 6,R2=0.922。结论:LPa-3单抗能实现自身配对,检测特异性好,是一种优良的体外诊断试剂盒原料,为胶乳增强免疫比浊试剂盒的开发奠定了基础。
Objective：Resolve the problem of Lp（a） antibody, such as the high variation between batches and can be affected by Kringle IV-2 polymorphism. Methods： Develop Lp（a） hybridomas and screen cell lines secreting antibodies matched itself and without reaction to Kringle IV-2. Antibody binding site and serum specificity were tested by indirect ELISA and sandwich ELISA, re- spectively. Results： Obtained a candidate hybridoma named LPa-3. LPa-3 monoclonal antibody recognize the Kringle IV-5 domain of Lp （ a）, and matched with itself in sandwich ELISA for serum Lp（a） detection. The comparative assay with 15 fresh serum samples in- dicates that LPa-3 sandwich ELISA with high correlation to a commercial immunoturbidimetric assay kit. The correlation equation and coefficient were y = 0. 190 4 x - O. 032 6 and R2 = O. 922, respectively. Conclusion： LPa-3 antibody, matched itself and unaffected by Kringle IV-2 polymorphism, will be an excellent candidate for the development of a latex-enhanced immunoturbidimetric assay method.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第4期505-507,519,共4页
Chinese Journal of Immunology
基金
北京博士后科研活动经费资助(No.2012ZZ-69)
