四种风湿病患者血小板参数变化及外周血BTLA、Treg观察

Observations changes of platelet parameters and BTLA,Treg in four kinds of rheumatic diseases

目的:观察四种风湿病患者血小板参数变化及外周血B、T淋巴细胞衰减因子(BTLA)、调节性T细胞(Treg)的表达。方法:采用流式细胞术检测风湿病患者外周血BTLA、Treg表达,生化仪检测血清血小板参数及其他实验室指标,观察风湿病患者血小板参数变化及与BTLA、Treg等的关系。结果:四种风湿病患者血小板异常率最高为RA,其次为AS、SS、OA;与正常组比较,这四种风湿病患者外周血BTLA、Treg表达降低;BTLA、Treg异常组血小板计数(PLT)、血小板压积(PCT)、血小板分布宽度(PDW)较BTLA、Treg正常组升高(P<0.05或P<0.01);血小板参数与BTLA、Treg呈相关性。结论:四种风湿病患者血小板异常可能与BTLA、Treg表达失衡有关。

Objective ：To observe the expression of B, T lymphocyte attenuator （BTLA）, regulatory T cells （Treg） and plate- let parameters in four kinds of rheumatic diseases. Methods： BTLA, Treg of peripheral blood were detected by flow cytometry, platelet parameters and other laboratory parameters of serum were observed using biochemical analyzer in patients with rheumatism. To observe the relationships between platelet parameters and BTLA, Treg. Results： The highest rate of rheumatic patients platelet abnormalities as RA, followed by AS, SS, OA. Compared with the normal group,the expression of BTLA, Treg were reduced in patients with rheumat- ic. Compared with BTLA, Treg of normal group, platelet count （PLT）, platelet hematocrit （PCT）, platelet distribution width （PDW） were increased in the abnormal group with BTLA and Treg （P 〈 O. 05 or P 〈 0. 01 ）. There were correlation between platelet parameters and BTLA, Treg. Conclusion： The platelet abnormalities maybe related to the imbalance of BTLA, Treg expression in the four kinds of rheumatic patients.