响应面法优化蔗梢多糖超声波提取工艺

Optimization on ultrasonic extraction of polysacchrides from sugarcane(Saccharum officinarum L.) tops by response surface analysis

【目的】优化蔗梢多糖的提取工艺条件,为蔗梢多糖的开发利用提供技术参考。【方法】以多糖提取率和DPPH自由基清除率为指标,采用响应面法分析优化超声波提取蔗梢多糖的工艺条件。【结果】超声波提取蔗梢多糖最佳工艺为:超声功率640 W,提取温度69℃,提取时间29 min,在此条件下,其多糖提取率为4.68%,DPPH自由基清除率为74.89%。【结论】采用响应面法优化蔗梢多糖超声波提取工艺具有较高的可行性。

[Objective]The extraction parameters of polysaccharides in sugarcane （Saccharum officinarum L.） tops were optimized to provide references for utilizing sugarcane top polysaccharides. [Method]With polysaccharides yield and DPPH scavenging activity as the indicators, response surface analysis （RSA） was used to optimize the ultrasonic extraction conditions for polysaccharides from sugarcane tops. [Result]The optimal conditions were ultrasonic power of 640 W, ex-traction temperature of 69 ℃, and extraction time of 29 min, under which the extraction yield and the DPPH scavenging rate of polysaccharides was 4.68% and 74.89%, respectively. [Conclusion]The response surface methodology was a rea-sonable method to optimize the extraction parameters of sugarcane top polysaccharides.