突托蜡梅叶化学成分及其乙酰胆碱酯酶抑制活性研究

The Study on Chemical Constituents and Acetylcholinesterase Inhibitory Activity of Petroleum Ether Extracts from Leaves of Chimonanthus grammatus

采用乙醇浸提法、液液萃取法得到突托蜡梅叶石油醚萃取物，用气．质联用法对其进行组成分析并用面积归一化法测定各化学成分的相对百分含量．使用改进了的Ellman比色法检测突托蜡梅叶石油醚萃取物对乙酰胆碱酯酶的抑制活性．突托蜡梅叶石油醚组分共鉴定了33个化学成分，其中螺[2，3]己烷．4—1，5，5-二乙基-6-甲基含量最高，占总质量的61．311％，其次是喇叭烯氧化物Ⅱ，占总质量的2．042％．突托蜡梅叶石油醚组分的乙酰胆碱酯酶抑制率在1～10mg·mL^-1范围变化明显，显示出一定的抑制活性，这为突托蜡梅叶的进一步开发利用提供了科学依据．

The petroleum ether extracts from leaves of C. grammatus were extracted from by ethanol extraction and liquid-liquid extraction, identified by GC-MS, and its AChE inhibitory activity were evaluated with the improved Ellman calorimetry. The petroleum ether extracts from leaves of C. grammatus contain 33 kinds of compounds, the major compounds include Spiro [ 2,3 ] hexan-4-one, 5,5-diethyl-6-methyl is highest （ 61.311% ） and Ledene oxide- （II） （2. 042% ）. However, the inhibition rate of AChE changed very obviously from 1 mg. mL^- 1 to 10 mg . mL^-1, which shows some inhibition activity. According to the result, it shows that petroleum ether fraction owns certain extent inhibition activity, which provides the experimental and theoretical basis to the further study on C. grammatus.