不同辅料对芒果苷在Caco-2细胞模型吸收转运的影响

Effect of different pharmaceutical excipients on Mangiferin Absorption and Transportation in Caco-2 Cell Model

[目的]研究不同的制剂辅料对芒果苷口服吸收的影响。[方法]采用人源结肠腺癌细胞系Caco-2细胞模型,考察制剂辅料(羟丙基-β-环糊精,聚乙二醇4000,聚乙二醇6000)对芒果苷在Caco-2细胞模型中吸收转运的影响。[结果]随着辅料浓度的增加,羟丙基-β-环糊精和聚乙二醇4000能改变芒果苷在Caco-2细胞模型中的渗透方向率PDR;而聚乙二醇6000则使芒果苷的渗透方向率PDR增大。[结论]羟丙基-β-环糊精和聚乙二醇4000能促进芒果苷的口服吸收;而聚乙二醇6000则无此作用。

[objective] To study the effect of different pharmaceutical excipient on mangiferin oral absorption. [Methods] Hu- man colon adenocarcinoma cell line （Caco-2 cell） was used as a cell model to investigate the effect of pharmaceutical excipients （hydroxypropyl-β-cyclodextrin, PEG4000, PEG6000）on absorption and transportation in Caco-2 cell model of mangifcrin. [ Results ] With the increase of excipients concentration, hydroxypropyl-β-cyclodextrin and PEG4000 could invert the permeability direction （PDR） of mangiferin in Caco-2 cell model, while PEG6000 increased permeability direction （PDR） of mangifein. [Conclusions] Hydroxypropyl-β-cyclodextfin and PEG4000 are beneficial to mangiferin absorption, while PEG6000 not.