摘要
目的:建立复方芦丁片中维生素C含量的测定方法。方法采用高效液相色谱法,0.02mol· L^-1乙酸铵溶液-甲醇(95∶5)为流动相;流速为1.0mL· min^-1;检测波长为267nm。结果维生素C的质量浓度在0.01~0.10mg· mL^-1范围内与峰面积呈良好的线性关系,r=0.9999,平均回收率为98.38%,RSD为0.96%。结论该方法简便、准确、重复性好,可用于测定复方芦丁片中维生素C的含量。
OBJECTIVE To establish a method for the determination of vitamin C in Compound Rutin Tab-lets.METHODS Determining the content of Vitamin C by HPLC ,the mobile phase was 0.02 mol· L^-1 ammonium acetate-methanol ( 95∶5 , v/v ) solution at a flow rate of 1.0 mL · min^-1;The detection wavelength was at 267nm.RESULTS The 1inear response range was 0.01~0.10mg· mL -1 of rutin(r=0.9999).The average re-coveries of vitamin C was 98.38%,RSD=0.96%.CONCLUSION The method is convenient ,accurate and repro-ducible.It can be suitable for the determination of vitamin C in Compound Rutin Tablets.
出处
《海峡药学》
2014年第4期66-68,共3页
Strait Pharmaceutical Journal