内寄生真菌Esteya vermicola对松材线虫侵染活力的测定

Infectivity Test on Esteya vermicola Conidia Against Pine Wood Nematode

从不同培养基、不同测定方法、不同菌株、不同孢子浓度4个方面测定内寄生真菌Esteya vermicola孢子对松材线虫的侵染活力,结果表明:该真菌离体分生孢子对松材线虫有侵染活力;PDA固体培养比PDB液体培养所得孢子能更有效的粘附和杀死松材线虫;在WA平板上,采用浸渍法测定孢子对线虫的侵染活力比其他处理效果好;在供试的3个菌株(CUN 120806、CBS 10082和CBS 115803)中,CBS 115803菌株对松材线虫具有较高侵染活性;4个不同浓度(1×109、1×108、1×107、1×106个·mL-1)孢子对线虫的作用中,随着孢子浓度降低,孢子对线虫侵染能力也随之降低。

To explore the infect effectiveness of Esteya vermicola conidia shed from the conidiogenous cells to pine wood nematodes,an infectivity test was conducted from different methods,different strains and different conidia concentrations.The results showed that although the conidia shed from the conidiogenous cells,they are still able to infect pinewood nematodes.In addition,the conidia had better effectiveness on pine wood nematodes by taking immersion method than other treatments.The strains CBS 115803 had higher infect effectiveness among 3 tested strains.The adherence and mortality rate fell with the decreasing of the initial conidia concentration.These results could be used as references for application conidia to control pine wood nematode and laid basis for further researches.