摘要
利用HPLC测定并比较全国各主要产地茯苓药材中茯苓酸含量.方法:采用waters symmetry C18(5μm,4.6×150mm)色谱柱,以乙腈和0.5%磷酸水溶液为流动相,分析测定了全国11个产地的茯苓药材中茯苓酸的含量.结果:在既定色谱条件下线性范围良好,该方法稳定性、重现性及茯苓酸加样回收率符合要求.鄂豫皖及云贵地区产地的茯苓药材茯苓酸含量高于其他产区,质量较好.结论:该方法准确、稳定,可为茯苓药材质量控制提供一定依据和科学保证.
To establish an HPLC method for determination of pachymic acid in poria cocos of different habitats. Methods： Determined the content of pachymic acid in poria cocos of 11 different habitats with waters symmetry C18 column（5lμm,4.6 × 150mm）, the mobile phase was acetonitrile and 0.5% phosphoric acid solution. Results： the content of pachymic acid in poria cocos of dabieshan area and Yunnan Guizhou province were significant higher than other habitats. Conclusion： A simple, accurate method is established for quality control of poria cocos.
出处
《通化师范学院学报》
2014年第4期42-44,共3页
Journal of Tonghua Normal University
基金
安徽省教育厅自然科学项目(KJ2011B076)
安徽中医药高专校级自然科学项目(ZRKX1019)
关键词
苓
茯苓酸
HPLC
poria cocos
pachymic acid
HPLC
