应用酵母双杂交系统筛选SlNAC3互作蛋白

Screening of Protein Involved in Interaction with SlNAC3 Using Yeast Two-Hybrid System

SlNAC3是负向调控番茄逆境胁迫的NAC蛋白.本研究应用酵母双杂交系统,以SlNAC3作为诱饵蛋白,通过筛选均一化的番茄cDNA文库,获得与SlNAC3相互作用的蛋白.通过对120个阳性克隆进行验证,最后得到了7个目标蛋白.其中包括细胞壁结构蛋白、转录相关蛋白以及乙烯信号传导途径相关蛋白等.从酵母双杂交的结果来看,SlNAC3在代谢领域,如核受体及其它细胞结构、转录因子的调节及信号传导等方面,可能都发挥非常重要的作用.

SlNAC3 is a NAC protein that negatively regulates tomato adversity stress.Interacting proteins of Sl-NAC3 are searched using yeast two-hybrid system by screening of homogenization of tomato cDNA library,u-sing SlNAC3 as the bait protein.Through the verification of the 120 positive clones,7 proteins interacting with SlNAC3 are filtered out,which include cell wall structural protein,transcription related protein and ethylene sig-nal transduction pathway related protein.The results reveal that SlNAC3 might play very important roles in non-metabolism field,such as nuclear receptors,cell structure,transcription factor regulation and signal transduc-tion.