RT-PCR方法检测烟草环斑病毒的研究

The Study on Detection Tobacco Ring-spot Virus (TRSV) by RT-PCR

根据烟草环斑病毒 (TRSV)外壳蛋白基因非编序列设计的引物P1,P2 ,用感病及健康组织总RNA为模板 ,进行cDNA合成和PCR试验 ,感病组织中扩增出了 6 0 0bp的目的片段 ,而健康组织中无此扩增带。摸索各项实验条件 ,建立了RT -PCR检测烟草环斑病毒 (TRSV)的方法。

A pairs of primers of P 1,P 2 were designed, corresponding the coat protein genes of TRSV. The cDNA was made, and PCR was gotten on, with total RNA of the infected tissue and health tissue. The target fragment about 600 bp was amplified from the infected sample, but not from health sample. More condition were tested, The method of detection of TRSV by RT-PCR was built.