高效液相色谱法检测蚕蛹油中主要脂肪酸的研究

Study on determination of main fatty acids in chrysalis oil by high performance liquid chromatography

建立了高效液相色谱-蒸发光散射检测器（HPLC—ELSD）同时检测蚕蛹油中三种主要脂肪酸的方法。以Hanbon Lichrospher C18柱（5μm，250mm×4．6mm）为固定相，丙酮-水（2％乙酸）（v：v）为流动相，通过梯度洗脱实现目标产物的基线分离。针对各目标物建立工作曲线，亚麻酸、棕榈酸和油酸的线性范围分别为0．2～2．0mg／mL、0．1～2．0mg／mL和0．2-2．5mg／mL，R2〉0．99，检出限（S／N=3）均为0．05mg／mL，回收率97％-107％，相对标准偏差均小于2％。该方法快速、简便，可同时检测蚕蛹油中三种主要的脂肪酸。

A rapid and simple method was established for simultaneous determination of three main fatty acids in chrysalis oil based on high performance liquid chromatography-evaporative light-scattering detection （HPLC-ELSD）. Chromatographic separation was carried out in gradient mode by using a Hanbon Lichrospher C18 column （5μm, 250mm ×46mm） with mobile phases of acetone-water （2% acetic acid ） （v/v）. The fatty acids were in baseline separated, respectively. The calibration curves were plotted on the double logarithmic scales. The linear ranges of linolenic acid,palmitic acid and oieic acid were 0.2-2.0g/mL,0.1 -2.0mg/mL and 0.2- 2.5mg/mL, respectively. R2〉0.99,the limits of detection （S/N =3） were 0.05mg/mL,the recoveries were 97% 107% and the relative standard deviations were below 2%. The analytical method established in this work could be applied to accurate and rapid determination of three fatty acids in chrysalis oil.