不同条件下副溶血弧菌生物膜形成规律及其天然抑制物质的研究

Study on the biofilm formation of Vibrio parahaemolyticus and the effect of its natural inhibitor under different conditions

主要研究了副溶血弧菌在不同培养务件下生物膜形成规律以及天然产物茶多酚、生姜提取物、大蒜提取物对其生物膜形成的影响。结果表明，不同副溶血弧菌菌株形成生物膜能力不同，其中VP—S17形成量最高。一般在培养48～72h时，生物膜达到成熟阶段，之后形成量开始下降。高温、高盐有助于提高副溶血弧菌生物膜的形成，3％盐浓度比1％浓度形成生物膜能力更强；盐浓度相同时，37℃条件下副溶血弧菌形成生物膜含量明显高于25％。茶多酚、大蒜提取物和生姜提取物能有效抑制副溶血弧菌生物膜的形成，较合适的抑制浓度分别为0．5％、1％和1％。

The biofilm formation of Vibrio parahaemolyticus under different culture conditions were studied and the effects of natural inhibitor such as polyphenols,ginger extract,garlic extract on biofilm formation were evaluated. The results showed that,the ability to form biofilm for Vibrio parahaemolyticus strains was different, and the VP-S17 was the hightest. The biofilm of Vibrio parahaemolyticus generally reached the maximum amount in 48~72h and then began to decline. High temperature and high salt were beneficial to improve the formation of biofilm. The amounts of biofilms under 1% salt concentration were less than 3% ,and when the salt concentrationsalt was the same,the formation of biofilm under 37℃ was significantly higher than 25℃. Polyphenols, garlic extract and ginger extract can effectively inhibit the biofilm formation of Vibrio parahaemolyticus, and the inhibitory concentrations were 0.5%, 1% and 1%, respectively.