摘要
为了研究鹧鸪茶提取物的抑菌活性及其物质基础,采用滤纸片法,以抑菌圈大小为评价指标,对鹧鸪茶提取物的抑菌性能进行测试,确定抑菌活性后,采用溶剂萃取法和离子沉淀法对鹧鸪茶提取物进行精制,并通过UV、GC、HPLC等方法分析鹧鸪茶提取物抑菌活性的物质基础。结果发现鹧鸪茶提取物对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌和酵母菌的抑菌作用较为明显,对其物质成分进行分析发现,鹧鸪茶提取物与茶多酚对照品存在共有色谱峰,茶多酚可能为鹧鸪茶提取物抑菌活性的物质基础之一。
Filtering paper method was used to determine the antimicrobial properties of mallotus oblongifolius by inhibitory zone.And the results showed that mallotus oblongifolius extraction had inhibitory effects on Bacillus subtilis, Escherichia coil, Staphylococcus aureus and Saccharomyces. The methods of solvent extraction and ion precipitation were adopted to purify the extract for the further analysis. Approaching the refined extract with GC,HPLC and UV scanning,and the tea polyphenol were proved to be the main antimicrobial component of mallotus oblongifolius.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第10期202-204,214,共4页
Science and Technology of Food Industry
基金
西安市科技计划项目(NC1207(2))
西安市未央区科技计划项目(201307)
陕西省农业厅农业推广计划项目(2013)
陕西省大学生创新创业训练计划项目(1068)
关键词
鹧鸪茶
提取物
抑菌活性
成分分析
mallotus oblongifolius
extraction
antibacterial activity
composition analysis
