马氏珠母贝肉酶解产物对SD大鼠生化指标的影响研究

Preliminary studies on physiological function of Pinctada martensii meat enzymatic hydrolysate

以马氏珠母贝肉为研究对象,采用正交实验对其酶解条件进行优化,并测定其酶解产物对SD大鼠血清生化指标的影响。结果表明:马氏珠母贝肉酶解的最优条件是酶用量0.6%、温度70℃、时间35min、pH8.0,在此条件下马氏珠母贝肉蛋白质水解度高达54.79%。马氏珠母贝肉酶解产物的调配试剂注射使SD大鼠血清中前白蛋白、载脂蛋白A1显著上升(p<0.05),谷丙转氨酶、谷草转氨酶、载脂蛋白B、肌酸激酶MB型同工酶、超敏C反应蛋白、葡萄糖、间接胆红素显著下降(p<0.05),结合相关研究结果,推测出马氏珠母贝肉酶解产物有保肝、保心血管的功效。

The enzymolysis technology of Pinctada martensii meat was optimized by orthogonal array design methods,and effect of the enzymatic hydrolysate on serum biochemical indicators in animal SD rat was investigated. The optimum enzymolysis conditions were found to be enzymolysis at 70℃ and pH8.0 for 35min with 0.6% dosage of enzyme,under the condition,degree of hydrolysis of Pinctada martensii meat protein reached up to 54.79%. After deployed reagent of Pinctada martensii meat enzymatic hydrolysate was injected into the animal SD rat. PA,ApoA1 in blood serum significantly rose（p〈0.05）,ALT,AST,ApoB,CK-MB,CRP, GLU, I-Bil in blood serum significantly dropped （p〈0.05）,therefore,combining with previous research results,it was inferred that Pinctada martensii meat enzymatic hydrolysate possessed medical functions of nourishing liver and cardiovascular.