以菊芋全粉为碳源培养基对乳杆菌的发酵性实验及发酵条件优化

Fermentable experiment and optimization of the fermentation conditions with Inulin Granules as carbon source culture medium

运用Design-Expert 8.0设计实验并以响应面分析的方法进行发酵条件优化。研究了分别以菊芋全粉、菊粉和葡萄糖作为培养基碳源,对保加利亚乳杆菌(ASI.1482)生长情况的影响,并证明了菊芋全粉良好的发酵性能。结果表明:菊芋全粉、菊粉作为单一碳源的培养基对乳杆菌具有增殖的作用,利用菊芋全粉对乳杆菌的增殖作用要优于菊粉和葡萄糖,乳杆菌的增殖数量分别为菊粉的4.8倍、葡萄糖的9.6倍,菊芋全粉作为乳杆菌培养基碳源成分具有可行性,其最优发酵条件为碳源添加量为2.1%,发酵温度为36℃,接种量为10%,在此条件下保加利亚乳杆菌的菌落总数为2.2×109CFU/mL。

Response surface method and the Design-Expert 8 were used to optimize the fermentation conditions. In the research,Inulin Granules, Inulin and Glucose were as the carbon source culture medium,which influenced the growth of the Lactobacillus bulgaricus（ASI.1482）,indicating that the excellent fermentation capability of the Inulin Granules. The results indicated that Inulin Granules or Inulin as the single carbon source media would have the proliferation effect on the lactobacillus,and this effect would be prior to that of the Inulin and Glucose with the lactobacillus proliferation of 4.8 times of Inulin and 9.6 times of Glucose, Inulin Granules was with the possibility of Lactobacillus bulgaricus carbon source culture medium. Its optimal fermentation conditions for carbon was 2.1% of source added volume,36℃ of fermentation temperature,and 10% vaccination rates. In such conditions,the count of Lactobacillus bulgaricus colonies was total for 2.2×109CFU/mL.