不同基因型大豆NADH-GOGAT活性动态规律研究

NADH-GOGAT Activities in Different Genotypes Soybean

以垦丰9号、东农46、东农42、黑农35、秣食豆和野生大豆为材料,研究不同基因型大豆全生育期的NADHGOGAT活性变化规律。结果表明:随着生育期的推进,不同基因型大豆叶片NADH-GOGAT的活性变化存在差异,野生大豆、半野生大豆呈单峰曲线,其他品种呈降-升-降趋势;茎秆NADH-GOGAT活性变化趋势基本一致,均呈单峰曲线;根瘤NADH-GOGAT活性变化东农46呈双峰曲线,其他品种呈单峰曲线;荚皮NADH-GOGAT活性各品种均呈下降趋势。不同器官NADH-GOGAT活性表现为叶片>根瘤>茎秆>根>荚皮。R4期叶片与茎秆中的GOGAT的活性与籽粒蛋白质含量呈极显著正相关。

Six varieties includiHg Heinong 35, Dongnong 42, Dongnong 46, Kenfeng 9, G. gracilis and G. soja were tested by means of pots experiments to study the dynamics of NADH-GOGAT activities in different genotypes soybean. The results indi-cated that during the whole growth stage, the dynamics of NADH-GOGAT activities in the leaves presented a drop-rise-drop tendency in G. gracilis and G. soja, the other varieties had a single-peak curve ; in stems it showed a single-peak curve; activities in roots of the tested varieties except Heinong 35 and Dongnong 42 showed a single-peak curve ;in the nodules of Dongnong 46 appeared a double-peak curve, but the other varieties had a single-peak curve;in pod decreased gradually with development. The gradation of GOGAT activities in different organs was leaf 〉 nodules 〉 stem 〉 root 〉 pod shed. The NADH-GOGAT activi-ties in leaves and stems were significant positively correlated with the protein content in seeds at R4.