草甘膦对不同基因型大豆不定芽再生的影响

Effect of Glyphosate on Adventitious Buds Induction from Embryonic Tip of Different Genotype Soybean

以5种大豆基因型的胚尖为外植体,研究了7种草甘膦浓度对大豆不同基因型胚尖不定芽形成的影响。结果表明:5种基因型大豆对草甘膦的耐性表现不同。吉育91耐性较强,草甘膦浓度为12.5 mg·L-1时不定芽诱导率、芽数、芽长显著低于对照;黑农37在草甘膦浓度为5.0 mg·L-1时不定芽诱导率、芽数显著低于对照,芽长在2.5 mg·L-1时显著低于对照;黑农35、黑农44、合丰35在草甘膦浓度为2.5 mg·L-1时胚尖不定芽的形成受到强烈抑制。

Embryonic tips from five soybean genotypes were used as the explants to investigate the effects of different concen- tration of glyphosate on the adventitious bud induction. The results showed that the tolerance from five soybean genotypes were different. In 12.5 mg·L^-1 glyphosate, the adventitious bud induction rate of Jiyu 91 were significantly lower than the control group;In 5.0 mg·L^-1 glyphosate, the adventitious bud induction rate and the number of the adventitious buds of Henong 37 were significantly lower than those of the control,who＇s the length of adventitious buds were lower than control groups in 2.5 mg·L^-1. In 2.5 mg·L^-1 glyphosate, the adventitious bud of Heinong 37, Heinong44 and Hefeng 35 were restrained strongly.