钙、铅处理对大豆根尖细胞有丝分裂的影响

Effect of Calcium and Lead Treatment on Mitosis of Soybean Root Tip Cell

以大豆（2n=40）品种黑河26为材料，研究了铅、钙及二者复合处理对大豆根尖细胞有丝分裂的影响。结果表明：铅、钙及其复合处理对大豆根尖细胞有丝分裂及染色体行为存在不同程度的影响。具体表现为：在0～100mg·L^-1。范围内，随Pb（NO3）2浓度增大染色体畸变率、微核率递增，有丝分裂指数则降低；0．1～1．0g·L^-1浓度Ca（NO3）2有利于植物根尖有丝分裂的进行，但大于2．5g·L^-1则具有抑制作用；铅钙复合处理条件下，钙在0．1—1．0g·L^-1浓度范围会缓解铅的毒害，钙的浓度过高反而会加重铅的毒性效应。

In this paper, we studied the effect of Pb, Ca and their combined treatments on root cell mitosis of soybean （2n = 40 ）euhivar Heihe 26. The results showed that with the concentration of Pb（ NO3 ）2 （0-100 mg·L^-1 ）increased the rate of chro-mosome aberration and micronueleus rate increased, and the mitotic index decreased. Lower concentration of Ca（ NO3 ）2 （0.1-1.0 g·L^-1 ） could enhance the plant root tip mitosis index, but inhibited that at high concentrations （ 2.5 and 4.5 g·L^-1 ）. In the Pb （ NO3 ）2 and Ca （ NO3 ）2 combined treatment, 0. 1-1. 0 g·L^-1 Ca （ NO3 ）2 could relieved the poison of 50 mg·L^-1 pb（N03 ）2.