摘要
目的了解泛耐药鲍氏不动杆菌(PDR-ABA)的菌株亲缘性。方法对20株PDR-ABA进行3种耐药相关看家基因(carO、gyrA、parC)和54种水平转移获得的与β-内酰胺类、氨基糖苷类和喹诺酮类药物耐药相关基因以及12种接合性质粒、转座子、插入序列、整合子等可移动遗传元件的遗传标记检测。结果 20株PDR-ABA中检出4种β-内酰胺类获得性耐药基因,5种氨基糖苷类获得性耐药基因,2种获得性抗茵制剂外排泵基因和5种可移动遗传元件。耐药相关看家基因carO、gyrA、parC均存在有义突变。结论 PDR-ABA存在5个克隆传播,其中1-3-4-5-9-15-16-18-20号菌株呈流行趋势。
Objective To conduct phylogenetic analysis in 20 strains of pandrug- resistant Acinetobacter baumanni （PDR- ABA）. Methods The analysis was completed in three drug resistance- related housekeeping genes （carO, gyrA, parC） and 54 species level shift obtained withβ- lactams, aminoglycosides, quinolones resistance- related genes as wel as 12 kinds of bonding nature of the particles, transposons, insertion sequences, integrons and other mobile genetic elements of genetic markers in 20 pan- resistant Acinetobacter baumanni . Results In 20 strains of PDR- ABA, 4 kinds ofβ- lactam- acquired drug resistance genes, 5 kinds of aminoglycoside acquired drug resistance genes, 2 kinds of acquired drug efflux genes, 5 kinds of removable genetic markers genetic components were detected. There were sense mutations in housekeeping genes carO, gyrA, parC. Conclusion The cluster analysis showed that five clones spread exist in sample group of strains, and the strains 1- 3- 4- 5- 9- 15- 16- 18- 20 have epidemic trend.
出处
《浙江医学》
CAS
2014年第8期663-665,共3页
Zhejiang Medical Journal
关键词
鲍氏不动杆菌
看家基因
水平转移基因
菌株亲缘性分析
Acinetobacter baumannii
Housekeeping gene
Gene acquired by horizontal transfer
Phylogenetic analysis
