四君子汤对脾虚证大鼠胃黏膜T细胞亚群的影响

Influence of Sijunzi decoction's on T lymphocyte subsets of gastric mucosa in rats with spleen-deficiency syndrome

[目的]观察四君子汤对脾虚证大鼠胃黏膜CD4+、CD8+T淋巴细胞的影响。[方法]102只SD大鼠随机分为正常对照组(24只)、长期造模组(26只)、自然恢复组(26只)和四君子汤组(26只)。正常对照组常规饲养7周,前4周给予生理盐水隔日1次灌胃,后3周每日1次灌胃(1.5ml/100g)。其余3组均采用复合因素造模法复制脾虚证大鼠模型,造模4周结束后,长期造模组继续造模3周,自然恢复组常规喂养3周,四君子汤组给予四君子汤每日1次灌胃3周(1.5ml/100g)。采用免疫组织化学法观察各组大鼠胃黏膜CD4+、CD8+T细胞的变化情况。[结果]与长期造模组和自然恢复组相比,四君子汤组大鼠胃黏膜CD4+T细胞数量增加,CD8+T细胞数量减少,差异有统计学意义(P<0.05)。四君子汤组CD4+T细胞数量的改善无法恢复到正常对照组水平(P<0.05),而CD8+T细胞的数量与正常对照组比较无明显差异(P>0.05)。[结论]四君子汤可明显增加胃黏膜CD4+T细胞数量,降低CD8+T细胞的数量,调节胃黏膜免疫功能,从而防治慢性胃病的发生和发展。

To investigate the influence of Sijunzi decoction on CD4＋ and CD8＋ T lymphocyte of gastric mucosa in rats with spleen-deficiency rats. [,Methods]One hundred and two SD rats were random- ly divided into four groups,i, e. ,the normal control group（n=26）,the long-term model group（n=26）,the natural recovery group（n=26）and the Sijunzi decoction group（n=26）. Rats in＇ the normal control group were raised in the routine way for 7 weeks and were given normal saline 1.5 ml per 100 g body weight by garage once every other day during the first 4 weeks and once everyday in the last 3 weeks. Spleen-deficien- cy rat model was made by composite factors in the other three groups. After the 4-weeks＇modelling time, the rats in long-term group were given modelling factors for another 3 weeks. Rats in the natural recovery group were raised in the routine way for 3 weeks. Rats in the Sijunzi decoction group were given Sijunzi de- coction 1.5 ml per 100 g body weight by gavage once everyday for 3 weeks. Immunohistochemistry was used to test CD4^＋ and CD8＋ T lymphocyte level in rats＇stomach tissue of every group. [Results]Compared with long-term model group and natual recovery group, the quantity of CD4^＋ T lvmohocvte in stomach tissue of Sijunzi decoction group increased, while the quantity of CD8＋ T lymphocyte decreased. The differ- ence was significant（P〈 0. 05）. The improve of CD4^＋ T lymphocyte in Sijunzi decoction group couldnot recover to the level of normal control group（P〈 0. 05）, while there was no difference in the quantity of CD8^＋ T lymphocyte between the Sijunzi decoction group and the normal control group（P〈0. 05）. [Conclu- sion]Sijunzi decoction can significantly increase the quantity of CD4^＋ T lymphocyte and decrease CD8^＋ T lymphocyte,and prevent the chronic stomach diseases by improving the gastric mucosal immunologic func- tion.