不同产地的景天三七中杨梅苷及没食子酸的含量测定

Determination of myricetrin and gallic acid in Sedum aizoon produced in different places

目的：采用高效液相色谱法（HPLC）分析不同产地景天三七中有效成分杨梅苷及没食子酸的含量。方法：以Hypresil BDSC18（4．6mm×250mm，5μm）为色谱柱，流动相分别为乙腈-0．1％磷酸溶液（22：78）、（1：99），流速1．0mL/min，柱温30℃；检测波长分别为256nm、273nm。结果：2种成分均在20min内得到较好分离，杨梅苷和没食子酸的进样量分别在0．0880—0．5280μg、0．2080-1．2480μg范围内与峰面积线性关系良好。平均加样回收率分别为99．4％、99．0％，RSD分别为1．29％、1．94％。结论：本方法简便，准确，重复性好，可作为景天三七的质量控制方法。

Objective：To analyze the content of myricetrin and gallic acid as effective components in Sedum aizoon produced in dif- ferent places by high- performance liquid chromatography （HPLC）. Methods： HPLC was performed on a Hypresil BDSC18（4.6mm×250mm,5μm） with a mobile phase of acetonitrile - 0. 1% phosphoric acid （22：78,1 ： 99） at a flow rate of 1.0 ml/min, a column temperature of 30℃, and detection wavelengths of 256 nm and 273 nm. Results ：Myricetrin and gallic acid were well separated within 20 min. The sample amounts of myricetrin and gallic acid showed a good linear relationship with peak areas within 0. 0880 - 0. 5280 μg and O. 2080 - 1. 2480 μg. The average recovery rates were 99. 4% （ RSD = 1.29% ） and 99. 0% （ RSD = 1.94）. Conclusion：This method is simple, accurate, and repeatable and can be used for the quality control of Sedum aizoon.