上海地区AIDS初诊病人合并感染CMV的CMV-gB基因分型

Genotyping of CMV-gB on initially diagnosed AIDS patients complicated with CMV infection in Shanghai

目的了解上海市艾滋病(AIDS)初诊病人合并巨细胞病毒(CMV)感染的CMV-gB基因分型特征。方法采用随机入组的方式,收集2011年3月至2012年2月,在上海市公共卫生临床中心就诊的AIDS初诊病人的血液,用套式聚合酶链反应(nPCR)确诊其是否感染CMV,并对阳性病人用限制性长度多态性分析(RFLP)法进行CMV-gB基因分型。结果在264名AIDS病人中,CMV-gB1型24例(占9.09%),gB2型0例,gB3型8例(3.03%),gB4型1例(0.38%)。CMV-gB基因阴性者与阳性者的CD+4T淋巴细胞计数差异有统计学意义(P<0.0001)。结论上海地区AIDS初诊病人中,CMV感染以CMV-gB1型为主,在CD+4T淋巴细胞计数<(108.4±22.18)/μL时需加强CMV的筛查。

Objective The study was to investigate Cytomegalovirus （CMV） gB genotyping features among ac quired immunodeficiency syndrome （AIDS） patients complicated with CMV infection. Methods With the method of randomly enrollment, blood samples of initially diagnosed AIDS patients were collected in the Shanghai public health clinical center from March 2011 to February 2011. Nested polymerase chain reaction （nPCR） was used to confirm CMV infection and positive patients were analyzed for CMV gB genotype with restriction fragment length polymor phism （RFLP） method. Results Among 264 enrolled AIDS patients, CMV gB1 type accounted for 9.09% （24/ 264）, gB2 type 0% （0/264）, gB3 type 3.03% （8/264）, and gB4 type 0.38% （1/264）. The difference of CD4 cell counts between CMV gB gene negative and positive patients was statistically significant （P 〈0. 0001）. Conclu sions In patients initially diagnosed as AIDS complicated with CMV infection in Shanghai, CMV gB1 is major. CMV screening needs to be strengthened in patients with CD4＋ T lymphocyte count below 108.4±22. 18/μL.