甘草甜素的提取工艺条件优化

The Extraction Process Optimization of Glycyrrhizin

以乙醇溶液作为提取溶剂,采用直接浸提回流法从甘草中提取甘草甜素。探讨提取温度、提取剂乙醇体积分数、料液比、提取时间4个因素对甘草甜素得率的影响,并通过正交试验确定甘草甜素的最佳提取工艺。结果表明,影响甘草甜素得率的因素按主次顺序由大到小依次为:B>D>C>A,即提取剂乙醇体积分数>提取时间>料液比>提取温度;最佳工艺条件的为A2B3C3D3,即提取温度50℃,提取剂乙醇体积分数30%,料液比1︰8(g/mL),提取时间270 min,在此工艺条件下甘草甜素的提取得率为27.2%。

Glycyrrhizin was extracted from Liquorice by refluxing extraction directly, using ethanol solution as extraction agent. The effects on glycyrrhizin extracted yield by the four factors of extraction temperature, exlraction solvent ethanol concentration, solid-liquid ratio and extraction time were discussed, and the best extraction process of glycyrrhizin was obtained though orthogonal test. The results showed that the primary and secondary descending order of the factors that affected glycyrrhizin yield as follows： B 〉 D 〉 C 〉 A, which meant extraction solvent ethanol concentration 〉 extraction time 〉 solid-liquid ratio 〉 extraction temperature. The best optimal process condition was A2B3C3D3, which the extraction temperature of 50 ℃, the extraction solvent ethanol concentration 30%, the solid-liquid ratio 1 ： 8 （g/mL）, the extraction time 270 min. The extraction yield of glycyrrhizin was 27.2% in this condition.