发酵豆芽中产物雌马酚的提取工艺研究

Study on Extraction Conditions of Equol Produced by Fermented Bean Sprouts

豆芽中含有丰富的异黄酮,其苷元形式在体内特定肠道菌的作用下可代谢为终产物雌马酚,雌马酚性质稳定,易被人体吸收,是大豆异黄酮生理功能的主要体现者。试验利用前期分离得到的产雌马酚菌株HY-1(屎肠球菌属)、菌HY-2(Slackia属),接种于豆芽粉中发酵,将豆芽粉中糖苷形式的异黄酮转化为苷元形式以及最终产物雌马酚,对产雌马酚菌株的应用性进行了初步探讨。通过单因素试验和正交试验确定从豆芽粉中提取雌马酚的最佳工艺,条件为乙醇体积分数80%,料液比1∶20(g/mL),提取温度为50℃,提取时间4 h。

Bean sprouts are rich in isoflavones, and the aglycone isoflavones can produce equol by the specific intestinal bacterial in v/vo. Equol is more stable, and absorbed more easily, in addition, the physiological activities of soy isoflavones is mainly reflected by equoL The aglycone isoflavones and the final metabolite equol were oblained from glucoside isoflavones of femaented bean sprout power, which was inoculated with Enterococcus faecium HY-1 and Slackia. HY-2. This experiment initiated us into the practicability of the equol-producing strains. Through single factor experiments and orthogonal experiments, it was proved that the op ＇tlmized extraction conditions of equol were ethanol concentration 80%, the ratio of solid-liquid 1 ： 20 （g/mL）, extraction time 4 h, the extraction temperature 50 ℃.