微波辅助提取反相高效液相色谱法测定花菜中的维生素C

Determination of Vitamin C in Cauliflower by Microwave Extraction and RP-HPLC

研究微波辅助提取，反相高效液相色谱测定花菜eevc含量的方法。花菜习浆后，以1％草酸为萃取溶剂，微波提取、净化后，采用恒流的方法进行液相色谱定量检测，以0．1％草酸溶液为流动相，流速1．0mL／min，采用C18反相色谱柱分离，在254nm波长下检测。结果表明，VC质量浓度在0．02-43．12mg／mL范围内线性良好，相关系数为0．9996，检测限0．02mg／kg,回收率在100．5％104．6％之间，相对标准偏差为1．20％。该方法能有效提取蔬菜中VC，测定结果稳定，重现性好，可以用于蔬菜中VC的检测。

The content of vitamin C in cauliflower was determined by microwave and HPLC. Vitamin C was extracted by microwave-assisted extraction of with 1% oxalic acid, then analyzed with HPLC by controlling the speed of mobile phase. Mobile phase was 0.1% oxalic acid with flow rate 1.0 mL/min; vitamin C was separated by C18 reversed column and detected by UV detector at 254 nm. It was showed that vitamin C in cauliflower could be separated. The linear ranges were 0.02-0.12 mg/mL, and the obtained correlation coefficient was 0.999 6. The recovery for the vitamin C ranged from 100.5%-104.6%, with the relative standard deviation （RSD） of 1.20%. The detection limits was 0.02 mg/kg. This method had good precision and high accuracy, thus can be applied to determine vitamin C in vegetables.