UPLC法同时测定丹参中11种成分的含量

Simultaneous Determination of 11 Components in Salvia miltiorrhiza by UPLC

目的:建立同时测定丹参中丹参素、原儿茶醛、咖啡酸、迷迭香酸、紫草酸、丹酚酸B、丹酚酸A、二氢丹参酮、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA11种化学成分含量的方法。方法:采用超高效液相色谱(UPLC)法。色谱柱为BEH C18(50 mm×2.1 mm,1.7μm),流动相为0.5%甲酸溶液-乙腈(梯度洗脱),流速为0.4 ml/min,检测波长为265 nm和280 nm。结果:该方法可在14 min内完成一次色谱分析,11种成分色谱峰之间有良好的分离度,各成分的质量浓度与各自峰面积积分值之间呈良好的线性关系,精密度、重复性、稳定性及加样回收率试验的RSD<2.0%。结论:该方法快捷、准确、重复性好,能同时测定丹参药材与饮片中11种成分的含量,可用于更全面地控制丹参的质量和丹参的相关研究。

OBJECTIVE： To establish a method for simultaneous determination of l 1 compounds in Salvia miltiorrhiza, such as tanshinol, protocatechuic aldehyde, caffeic acid, rosmarinci acid, lithospermic acid , salvianolic acid B, salvianolic acid A, tan- shinone I , cryptotanshinone, dihydrotanshinone, tanshinone 11 A. METHODS： UPLC method was adopted. The determination was performed on BEH C18（50 mm×2.1 ram, 1.7 μm） column with mobile phase consisted of 0.5% methanoic acid-acetonitrile （gradi- ent elution） at the flow rate of 0.4 ml/min. The detection wavelength was set at 265 nm and 280 nm. RESULTS： 11 components were separated clearly by this method within 14 rain. A good linearity was obtained between the concentration and the peak areas of 11 compounds. RSDs of precision, reproducibility, stability and recovery tests were all less than 2.0%. CONCLUSIONS： The method is rapid, accurate and reproducible, and can be used for simultaneous determination of 11 compounds in S. miltiorrhiza and decoction pieces. It can be used for quality control and relative study of S. miltiorrhiza.