苦参生物碱类成分的HPLC指纹图谱研究

Study on HPLC Fingerprints of Alkaloids from Sophora flavescens

目的:建立苦参生物碱类成分的高效液相色谱(HPLC)指纹图谱。方法:色谱柱为Kromasil NH2(250 mm×4.6 mm,5μm),流动相为乙腈-乙醇(8∶1,V/V)-3%磷酸水溶液(梯度洗脱),流速为1 ml/min,检测波长为220 nm;采用《中药色谱指纹图谱评价系统》(2004A版)对3批样品进行相似度评价。结果:建立的HPLC指纹图谱中,苦参生物碱类成分各峰分离度较好,一共确定了17个共有峰,并对其中5个峰进行了定位指认;3批样品的相似度均>0.9。结论:该方法可得到精密度、重复性、稳定性较好的苦参生物碱类成分的HPLC指纹图谱,为苦参提取物的质量控制提供另一种检测方法。

OBJECTIVE： To establish HPLC fingerprint of alkaloids from Sophora flavescens. METHODS： The determination was performed on Kromasil NH2（250×4.6 ram, 5 μm） column＇with mobile phase consisted of acetonitrile-ethanol （8： 1, V/V）-3% phosphoric acid （gradient elution） at the flow rate of 1 ml/min. The detection wavelength was set at 220 nm. The similarity of 3 batches of samples was evaluated by using TCM Chromatographic Fingerprint Evaluation System （2004 A edition）. RESULTS： In HPLC fingerprints, the chromatographic peaks of alkaloids from S. flavescens were well-separated, and there were 17 common peaks, among which 5 peaks were positioned and identified. The similarity of 3 batches was more than 0.9. CONCLUSIONS： HPLC fingerprint of alkaloids from S. flavescens can be obtained with good precision, reproducibility and stability, providing anoth- er detection method for quality control of alkaloids from S. flavescens.