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不同阴离子组成的离子液体对产紫青霉生长、代谢及催化特性的影响 被引量:4

Effects of Ionic Liquids with Different Anion Composition on Cell Growth,Metabolism and Catalysis Characteristics of Penicillium purpurogenum Li-3
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摘要 研究了6种不同阴离子分别与1-丁基-3-甲基咪唑阳离子[Bmim]+组成的6种离子液体对产紫青霉(Penicillium purpurogenum Li-3)细胞生长、代谢、细胞膜透性和催化活性的影响.结果表明,[Bmim]Ac和[Bmim]Tf2N对菌体细胞的生长和代谢具有明显的抑制作用;6种离子液体均改善了细胞膜透性,其中以[Bmim]Ac最明显;全细胞催化反应实验表明,在分别含25%的疏水性离子液体[Bmim]PF6和[Bmim]Tf2N的2种介质体系中,甘草酸(GL)生物转化合成单葡萄糖醛酸基甘草次酸(GAMG)的转化率分别为82.73%和89.84%,GAMG的产率分别为68.65%和76.23%,与不含离子液体的纯缓冲溶液体系相比(GL转化率和GAMG产率分别为81.82%和33.31%),GL的转化率均略有提高,而GAMG的产率则提高了2倍多,表明2种疏水性离子液体对全细胞催化反应的定向性具有明显促进作用;亲水性离子液体[Bmim]Ac,[Bmim]Cl和[Bmim]NO3对细胞的催化活性具有明显的抑制作用,而[Bmim]BF4的抑制作用则较弱. The wild-type Penicillium purpurogenum Li-3 strain is capable of expressing highly β-glucuroni-dase, a intracellular enzyme with excellent ability to biotransform glycyrrhizin(GL) into glycyrrhetinic acid monoglucuronide ( GAMG ) possessing best biological function. To improve the production of GAMG, the effects of ionic liquids with different anion composition on Penicillium purpurogenum Li-3 cells properties of growth, metabolism, permeability of cell membrane and catalysis were studied in this paper. The results showed that [ Bmim] Ac and [ Bmim] Tf2 N had obvious inhibitory effects on cell growth and metabolism. Six kinds of ionic liquids all improved the permeability of cell membrane, and [ Bmim] Ac was the most remarka-ble. In the two catalytic medium respectively containing 25% hydrophobic [Bmim]PF6 and [Bmim]Tf2N, conversion rates of substrate GL were 82. 73% and 89. 84%, and the yields of GAMG were 68. 65% and 76. 23%, respectively. Thus, the substrate GL conversion increased slightly, but the yield of GAMG were about two times higher than that in the pure buffer solution without ionic liquids ( conversion rate of GL 81. 82%, yield of GAMG 33. 31%) . It was obvious that hydrophobic [ Bmim] PF6 and [ Bmim] Tf2 N were ca-pable of promoting the selectivity of the whole cell catalysis. While hydrophilic [ Bmim] Ac, [ Bmim] Cl and [ Bmim] NO3 obviously had inhibition in different degrees on the cell’ s catalytic activity. These results indica-ted that the anions in ionic liquids played a crucial role as biological catalytic medium.
出处 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2014年第5期1057-1062,共6页 Chemical Journal of Chinese Universities
基金 国家自然科学基金项目(批准号:21146012 21266029) 教育部科学技术研究重点项目(批准号:209146) 新疆生产建设兵团博士资金(批准号:2009JC13) 石河子大学高层次人才科研启动资金专项(批准号:RCZX200805)资助~~
关键词 离子液体 阴离子 产紫青霉 全细胞 催化特性 Ionic liquid Anion Whole cell Catalytic property
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  • 1龚珍,李象远,李泽荣.水和甲醇混合溶剂氢键相互作用及对高分子链溶解能力的理论研究[J].高等学校化学学报,2004,25(3):539-542. 被引量:8
  • 2许金钩,王尊本.荧光分析法.北京:科学出版社,2006:67
  • 3荣杰峰, 苏志明, 张甜真, 林涛, 陈清梅, 蔡志雄, 池毓务. 分析化学, 2009, 37(21), 31.
  • 4Jia X. D., Han S. Y., Duan H. F., Lin Y. J., Cao J. G., Liang D. P., Wu M. C., Chem. Res. Chinese Universities, 2013, 29(1), 82-86.
  • 5Yan R., Shao M. Y., Liang Z. F., Zhang H. Q., Yu A. M., Chem. Res. Chinese Universities, 2013, 29(2), 218-222.
  • 6Simon R., Kuhnel., Balducci A., J. Phys. Chem. C, 2014, 118, 5742-5748.
  • 7Shao B. B., Shi L., Meng X., Ind. Eng. Chem. Res., 2014, 53, 6655-6663.
  • 8Faria R. A., Ponte M. N. D., Bogel-Lukasik E., Fluid Phase Equilibria, 2014, (385), 1-9.
  • 9Pang N., Gu S. S., Wang J., Bioresource Technology, 2013, 139, 337-342.
  • 10Yang M. Y., Wu H., Lian Y., Li X. F., Ren Y., Lai F. R., Zhao G. L., Microbial Cell Factories, 2014, 13, 143-153.

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