HPLC法测定复方黑参颗粒中肉桂酸与哈巴俄苷含量

Determination of Cinnamic Acid and Harpagoside in Fufang Heishei Granules by HPLC

目的：建立复方黑参颗粒中肉桂酸和哈巴俄苷的含量测定方法。方法：采用高效液相色谱法,色谱柱Dikma Diamonsil-C18（4.6 mm×250 mm,5μm）,流动相：甲醇-0.5%甲酸水溶液（56∶44）等度洗脱,流速1.0 mL.min-1,柱温25℃,检测器波长278 nm。结果：肉桂酸和哈巴俄苷进样量分别在1.53-40.80、4.56-121.60μg.mL-1时,线性关系良好,线性范围依次为Y=190177X-39502（r=0.9997）;Y=55216X-21379（r=0.9996）。平均加样回收率分别为99.6%（RSD1.0%）,100.8%（RSD1.2%）。结论：样品处理方法简便、结果准确、重复性良好,该方法可用于复方黑参颗粒的质量控制。

Objective： To establish an HPLC method for the determination of Cinnamic acid and Harpagoside in Fufang Heishei Granules. Method： The Dikma Diamonsil-Cis column was used with a mobile phase of methanol-0.5% ice acetic （ 56：44 ）. The flow rate was 1 mL · min-1. The column temperature was 25 ℃ . The detection wavelength was at 278 nm. Result ： The calibration curves of Cinnamic acid and Harpagoside were linear in the ranges of 1.53-40.80,4.56-121.60 μ g · mL-1 respectively. And the each average recoveries of the method were 99.6% （ RSD1.03% ）, 100.8% （ RSD1.25% ）. Conclusion ： The method is simple, accurate and repeatable. It can he used for quality control of Fufang Heishei Granules.