合成红景天苷对H9c2细胞氧化应激损伤的保护作用研究

Protective Effects of Synthetic Salidroside on H9c2 Cardiomyocytes Oxidatively Injured by H_2O_2

目的:研究合成红景天苷对过氧化氢所致H9c2心肌细胞氧化应激损伤的保护作用。方法:体外培养H9c2细胞,分为正常对照组、模型组、提取红景天苷(10-4 mol·L-1)组和合成红景天苷(10-6、10-5、10-4mol·L-1)组,药物孵育24 h后,加入过氧化氢终浓度400μmol·L-1损伤4h,检测各组细胞活力和培养液上清液中乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量。结果:与正常对照组相比,模型组细胞活力、SOD活性显著降低,LDH、CK活性和MDA含量显著升高(P<0.01)。与模型组相比,红景天苷预处理组能明显提高细胞活力和SOD活性(P<0.05),明显降低LDH、CK活性及MDA含量(P<0.05),并与浓度呈正相关。结论:红景天苷对过氧化氢造成的心肌细胞氧化应激损伤有明显的保护作用,且合成红景天苷效果优于提取红景天苷。

Objective：To investigate the protective effects of Synthetic Salidroside（Syn Sal） on H9c2 cardiomyocytes after oxidative stress of H2O2.Methods：H9c2 cardiomyocytes were cultured in vitro and divided into normal control group,model group,Extractive Salidroside（Ext Sal,10-4mol·L-1） pretreatment group and Synthetic Salidroside（Syn Sal,10-6,10-5,10-4mol·L-1） pretreatment groups.H9c2 cells were pretreated with vehicle or different doses of Sal for 24 h and then incubated with control or 400 μmol·L-1 H2O2for another 4 h,then the cell viability,the activities of LDH,CK and SOD and the content of MDA were measured.Results：Compared with the normal control group,the viability of H9c2 cells and SOD were decreased,the activities of LDH and CK and the content of MDA were increased significantly（P＆lt; 0.01） in the model group.Compared with the model group,the viability of H9c2 cells and SOD were increased significantly,the activities of LDH and CK and the content of MDA were decreased significantly（P＆lt;0.05） in Sal pretreatment groups,which was positively related with drug dosage.Conclusion：Salidroside pretreatment can obviously protect H9c2 cardiomyocytes from oxidative injury in a concentration-dependent manner,and the effect of Synthetic Salidroside is better than the Extractive Salidroside.