假单胞菌碳代谢抑制调控系统各组分的功能及作用机制

Function and Action Mechanisms of Each Component in Inhibition and Regulation System in Pseudomonas

在复杂的生存环境中,微生物通过碳代谢抑制作用(CCR)优先利用优势碳源,同时抑制非优势碳源的使用,进而达到最佳的生长和代谢过程。碳代谢抑制调控在细菌中普遍存在,但在不同物种间存有差异。大肠杆菌中以CRP蛋白为核心,而假单胞菌的碳代谢抑制调控以CbrAB-CrcZ-Crc为核心发挥作用,其中Crc蛋白是一个全局性的调控因子,CrcZ是一个新发现的非编码调控RNA,二元调控系统CbrAB控制CrcZ的表达,整个过程的调控复杂且高效。将国际上最新的相关进展与本实验室工作相结合,详细介绍了假单胞菌属中CbrAB-CrcZ-Crc调控系统的作用机制,有助于提高对微生物碳代谢抑制调控以及环境适应机制的认识。

In the complex environment, microorganism select to use the perferred carbon source by carbon eatabolite repression（CCR） and at the same time inhibit the non-perferred carbon sources, so as to achieve the best growth and metabolism procedure. This phenomenon is widespread in bacteria, but there exist differences among different species. In Escherichia coli, CRP protein plays the core role, while in Pseudomonas, the CbrAB-CrcZ-Crc system is critical. Crc protein is a global regulator. CrcZ is a newly identified non-coding regulatory RNA, whose expression is regulated by 2 component system CbrAB. The whole regulatory process is under complex and efficient control. Combining the related international recent progresses and our laboratory work, this paper introduced in detail the regulation mechanism of CCR by CbrAB-CrcZ-Crc regulation system in Pseudomonas, expected to improve our awareness about the regulatory mechanism of carbon metabolism and microorganism＇s adaptability to environment.