“通利大肠”对COPD模型大鼠肺组织SP、VIP含量的影响

Effect of Relaxing Large Intestine Therapy on Contents of SP and VIP in Lung Tissues of Rat Models with Chronic Obstructive Pulmonary Diseases

目的:观察"通利大肠"对慢性阻塞性肺疾病(COPD)模型大鼠肺组织P物质(SP)、血管活性肠肽(VIP)含量的影响,从神经肽角度探讨COPD"从肠论治"的效应机制。方法:采用熏烟联合气管注射脂多糖复制COPD大鼠模型。50只雄性大Wistar鼠随机分为正常组、模型组、治肠组、治肺组、肺肠同治组。正常组和模型组均灌胃给予生理盐水,治肠组、治肺组、肺肠同治组分别用治肠药(生大黄)、治肺药(生石膏、苦杏仁、瓜蒌皮)、肺肠同治药(生大黄、生石膏、苦杏仁、瓜蒌皮)灌胃,连续7 d。用免疫组织化学法和酶联免疫吸附法检测肺组织中SP、VIP含量。结果:免疫组化结果显示,与正常组相比,模型组大鼠肺组织SP表达较强,而治肠组、治肺组、肺肠同治组表达较弱;VIP在正常大鼠支气管上皮表达较强,模型组表达明显减弱,各治疗组VIP表达明显增强。酶联免疫吸附法结果显示,与正常组相比,SP在模型组肺组织中含量明显升高,VIP含量明显降低(P<0.01);与模型组相比,治肠组、治肺组、肺肠同治组肺组织SP含量均明显降低,VIP含量明显升高(P<0.01或P<0.05);与治肺组相比,肺肠同治组肺组织SP含量明显降低(P<0.05)。结论:通利大肠或治肺基础上增加通利大肠,可调节COPD模型大鼠肺组织神经肽SP、VIP的含量,这可能是COPD"从肠论治"的效应机制之一。

Objective：To observe the influence of relaxing large intestine therapy on contents of substance P （SP） and vasoactive intes-tinal peptide （VIP） in rat model with chronic obstructive pulmonary disease （COPD） and explore the effective mechanism of treating COPD based on relaxing the large intestine from the aspect of neuropeptide .Methods：Rats model of COPD were established through in-tratracheal injection of LPS combined with cigarette smoking .Fifty Wistar rats were randomly divided in to normal group , model group , intestine group , lung group and lung-intestine group .The normal group and model group were intragastrically given normal saline solu-tion and other groups were treated with corresponding decoction intragastrically for 7 days.The content of SP and VIP were detected by immunohistochemistry and enzyme-linked immunosorbent assay .Results：Immunohistochemistry results showed that compared with con-trol group , SP in lung tissue of model group was abundantly expressed while in lung group and intestine group and lung -intestine group were weakly expressed .VIP in bronchial epithelium of control group was expressed broadly while model group were lower .After treat-ment , VIP was increased significantly in trial groups .Enzyme-linked immunosorbent assay showed that compared with control group , SP was increased in lung tissue in model group while VIP were decreased significantly （ P＆lt;0.01 ） .Compared with model group , SP was lower in lung group, intestine group and lung-intestine group.VIP was increased significantly （P＆lt;0.01 or P＆lt;0.05）.Compared with lung group, SP was reduced significantly in lung-intestine group （P＆lt;0.05）.Conclusion：The expression of SP and VIP were regulated by the therapy of relaxing the large intestine or relaxing the large intestine on the base of treating lung disease .This may be one of the mechanisms of why treating from intestine is effective on COPD .