针刺对骨骼肌急性钝挫伤模型形态学影响的实验观察

Observation on Experiment of the Morphological Effects of Acupuncture on Acute Blunt Skeletal Muscle Contusion Model

目的:观察针灸针斜刺阿是穴对家兔腓肠肌急性钝挫伤修复的形态学影响。方法:采用定量负荷自由落体复制肌肉急性闭合性钝挫伤的方法对模型家兔右后肢腓肠肌进行造模,分别于造模后即刻、造模后24 h、造模后48 h测量腓肠肌肿胀度,肌肉硬度,于造模后48 h取材,并检测血清中肌酸激酶、乳酸脱氢酶含量,并通过HE染色光镜和透射电镜观察家兔腓肠肌形态学改变。结果:造模后48 h各组家兔血清肌酸激酶(CK)含量比较,与正常组相比,模型组、针刺组、CsA模型组、CsA针刺组均显著身高(P<0.05),与模型组相比,CsA模型组显著升高(P<0.05);造模后48 h各组家兔乳酸脱氢酶(LDH)含量,与空白组比较,模型组和CsA模型组显著升高(P<0.05),与模型组相比,针刺组和CsA针刺组显著降低(P<0.05);HE染色光镜下,模型组及各干预组可见不同程度的肌纤维损害;透射电镜下模型组及各干预组可见不同程度的超微结构变化。结论:本实验中的造模方法可以成功复制腓肠肌急性闭合性钝挫伤模型,针灸针斜刺阿是穴可有效地促进腓肠肌急性钝挫伤修复,环孢素干预一定程度降低了针刺的修复作用。

Objective： To observe the morphological effects of acupuncture on Ashi points of rabbits with acute gastrocnemius blunt con- tusion. Methods： Modeling was given on gastrocnemius muscle in rabits hindquarter by using method of free falling with quantitative load to duplicate acute skeletal muscle injury. The swelling degree and hardness of gastrocnemius（ immediately, 24hours, 48hours after building the model） was measured. Materials were taken 48hours after building the model. The content of CK and LDH in serum were tested. The morphological changes of gastrocnemius under light microscope and electron microscope were observed. Results： 48 hours after building the model, the content of CK of model group, acupuncture group, CsA model group and CsA acupuncture group increased significantly compared the nature group （ P 〈 0. 05 ） ; compared with the nature group, differences of the content of LDH of model group and CsA model group increased significantly （P 〈 0. 05 ） ; compared with the model group, acupuncture group, CsA model group and CsA acupuncture group decreased significantly （ P 〈 0. 05）. Under light microscope, different kinds of muscle fiber trauma could be found in all groups except the nature group. Under electron microscope, Ultrastructural changes could be seen in all groups except the nature group. Conclusion： The modeling method can successfully copy acute closed skeletal gastrocnemius muscle injury model. Acu- puncture could promote the reparative process of acute blunt muscle effectively, while CsA would partially reduce the repairing effect of acupuncture.