不同产地加工方法对野菊花中三种活性成分含量的影响

Effects of Different Processing Methods on the Contents of Three Active Ingredients in Flos Chrysanthemi indici

考察了不同产地加工方法对野菊花中蒙花苷、绿原酸和3，5-二咖啡酰奎尼酸3种活性成分的影响，以高效液相色谱法测定其含量。采用DiamonsilC18色谱柱，以乙腈：0．2％磷酸为流动相，梯度洗脱，检测波长330nm。结果蒙花苷、绿原酸和3，5-二咖啡酰奎尼酸分别在0．08～0．8、0．046～0．46和0．066～0．66Pg范围内线性关系良好。其回收率分别为98．9％、98.3％和98．1％，RSD分别为2．07％、1．47％和1．19％。

The effects of different processing methods on the contents of buddleoside, chlorogenic acid and 3,5-O-dicaffeoylquinic acid in Flos Chrysanthemi indici were investigated and their concentrations were determined by HPLC. A Diamonsil C18 column was used, with the mobile phase of acetonitrile ： 0.2% phosphoric acid by gradient elution, at the detection wavelength of 330 nm. The calibration curves for buddleoside, chlorogenic acid and 3,5-O-dicaffeoylquinic acid were linear in the ranges of 0.08 - 0.8, 0.046 - 0.46 and 0.066 - 0.66 μg. Their recoveries were 98.9%, 98.3 % and 98.1%, with RSDs of 2.07%, 1.47% and 1.19%, respectively.