摘要
目的研究纳米二氧化钛(TiO2)对大鼠的氧化应激损伤效应,以及蜂胶对这种损伤的防护效果。方法采用无特定病原体级雄性SD大鼠35只,随机分为7组,每组5只。低、中、高剂量染毒组分别灌服给药剂量为8、16、32 mg/(kg体质量·d)纳米TiO2溶液,共7 d;低、中、高剂量蜂胶干预组于灌服0.2 g/(kg体质量·d)蜂胶后,间隔4 h,再分别灌服8、16、32 mg/(kg体质量·d)纳米TiO2溶液,灌服7 d后,中、高剂量蜂胶干预组分别继续灌服0.2g/(kg体质量·d)蜂胶7和14 d;对照组大鼠仅灌服灭菌生理氯化钠溶液2 ml,共7 d。采集各组大鼠下腔静脉血,分离血清,采用分光光度法测定血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)水平。结果与对照组比较,中、高剂量染毒组SOD活力下降[(1 187.0±5.7)vs(1 215.2±21.7)×103μmol/(min·L),(1 154.4±10.4)vs(1 215.2±21.7)×103μmol/(min·L),P<0.05],MDA水平增加[(1.71±0.03)vs(1.62±0.07)μmol/L,(1.78±0.03)vs(1.62±0.07)μmol/L,P<0.05]。大鼠血清中SOD活力随着纳米TiO2剂量的增加而降低[回归方程:^y=-1.820 x+1 213.000,决定系数(R2)=0.748,P<0.01],MDA水平随着纳米TiO2剂量的增加而增加[回归方程:^y=0.005 x+1.630,R2=0.656,P<0.01]。蜂胶干预处理大鼠的SOD活力高于染毒处理大鼠(P<0.01);蜂胶干预处理大鼠的MDA水平低于染毒处理大鼠(P<0.01)。结论在本实验条件下,纳米TiO2可引起大鼠的氧化损伤,蜂胶能够增强大鼠的抗氧化应激能力。
Objective To explore the protective effect of propolis on redox damage to blood induced by nano -TiO2 in rats. Methods Thirty-five specific pathogen free healthy male SD rats were randomly divided into seven groups , with five rats in each group.The low-, median-and high-exposure groups were gavaged with nano-TiO2 at daily doses of 8, 16, and 32 mg/kg body weight （ bw） for 7 days respectively .The low-, median-and high-propolis intervention groups were gavaged with propolis at daily doses of 0.2 g/kg bw first, then 4 hours later gavaged with nano-TiO2 at daily doses of 8, 16, and 32 mg/kg bw.After being treated with propolis and nano-TiO2 for 7 days, the median-and high-propolis intervention groups were continuously gavaged with propolis at a daily dose of 0.2 g/kg bw for 7 and 14 days, respectively.The control group was gavaged with 2 ml sterilized normal saline for 7 days.The activity of superoxide dismutase （SOD） and the level of ma-lonic dialdehyde （ MDA） in blood serum were determined using the spectrophotometric method .Results Compared to the control group, the activities of serum SOD in median-and high-exposure groups were significantly lower [（1 187.0 ＆#177;5.7） vs （1 215.2 ＆#177;21.7） ＆#215;103 μmol/（min＆#183; L）, （1 154.4 ＆#177;10.4） vs （1 215.2 ＆#177;21.7） ＆#215;103 μmol/（min＆#183; L）, P＆lt;0.05], and the levels of MDA were significantly higher [（1.71 ＆#177;0.03） vs （1.62 ＆#177;0.07） μmol/L, （1.78 ＆#177;0.03） vs （1.62 ＆#177;0.07） μmol/L, P＆lt;0.05].SOD activity in serum of the exposure groups significantly decreased with the dose＆amp;nbsp;[the regression equation was y^=-1.820 x＋1 213.000, coefficient of determination （R2） =0.748, P＆lt;0.01）, and the level of MDA significantly increased with the dose （ the regression equation was y^=0.005 x＋1.630, R2 =0.656, P＆lt;0.01）.SOD activity in serum of the propolis intervention groups was significantly higher than those of exposure groups （P＆lt;0.01）, and MDA levels in serum of the propolis intervention groups were significantly lower than those of exposure groups （P＆lt;0.01）.Conclusion Under the experimental conditions , nano-TiO2 can lead to disturb the redox state in rats persistently, and propolis shows some protective effects on redox damage .
出处
《中国职业医学》
CAS
北大核心
2014年第2期147-150,共4页
China Occupational Medicine
基金
建设中医药强省科研课题(20121267)
